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INDIVIDUAL VARIATION OF MUCIN CONCENTRATION IN HUMAN SALIVA

INDIVIDUAL VARIATION OF MUCIN CONCENTRATION IN HUMAN SALIVA. P.A. Denny, W. Wu, and P.C. Denny School of Dentistry, University of Southern California Los Angeles, USA

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INDIVIDUAL VARIATION OF MUCIN CONCENTRATION IN HUMAN SALIVA

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  1. INDIVIDUAL VARIATION OF MUCIN CONCENTRATION IN HUMAN SALIVA P.A. Denny, W. Wu, and P.C. Denny School of Dentistry, University of Southern California Los Angeles, USA Data reported in part in “A Blind Study of MG1 and MG2 Mucin Concentrations in Repeated Collections of Whole Saliva from Young Subjects” by W. Wu, a Master of Science degree thesis for the Craniofacial Biology Program, School of Dentistry, USC 1993

  2. I. Introduction • The study presented in P.C. Denny, P.A. Denny, D.K. Klauser, S.H. Hong, M. Navazesh, and L.A. Tabak. “Age-related Changes in Mucins from Human Whole Saliva”, J Dent Res. 70:1320-1327 (1991) revealed that MUC7 mucin concentration in a single collection of unstimulated whole saliva from each individual ranged from <6 units/ml to 1500 units/ml in a group of young adults 18-33 years old. • We subsequently pursued the question whether this broad range of mucin concentrations was a reflection of the range of variation within each individual or a reflection of the range of individual differences. • Our approach was to select six new individuals from the same age category whose MUC7 mucin concentrations in one collection of unstimulated saliva ranged from <100 units/ml to 1500 units/ml. • Repeated collections of both unstimulated and stimulated whole saliva were obtained at a minimum of weekly intervals over a period of three months. Flow rates, and concentrations of MUC7 mucin and MUC5B mucin were obtained for each sample.

  3. II. Quantitation of MUC7 and MUC5B Mucins (from Denny et al., J Dent Res. 70:1320-1327 (1991)) The two mucins in whole saliva were fractionated by SDS-PAGE, stained with Stains-all, photographed in black and white using a red filter, and quantitated by densitometry. Mouse Muc10 Whole Human Saliva MUC5B MUC7 MUC5B MUC7 1.25 2.5 5 10 20 40 Mucin Units Mucin Units There was no detectable loss of either mucin under our conditions for collection, storage, assay and re-assay of the same sample. Neuraminidase removes all Stains-all reactivity with MUC7 mucin and approximately 50% of reactivity with MUC5B mucin (L.W. Baughan, F.J. Robertello, D.C. Sarrett, P.A. Denny, and P.C. Denny. “Salivary Mucin as Related to Oral Streptococcus mutans in the elderly”, Oral Micro. Immunol. 15:10-14 (2000)).

  4. IV. Individual and Population Variation of MUC7 Mucin Concentrations in Repeated Collections of Unstimulated Saliva Two-level Nested Analysis of Variance (nANOVA)

  5. VI. Individual and Population Variation of MUC5B Mucin Concentrations in Repeated Collections of Unstimulated Saliva Two-level Nested Analysis of Variance (nANOVA)

  6. VII. Means Test for Repeated Collections of Unstimulated Whole Saliva MUC7 mucin MUC5B mucin 0 250 500 750 1000 1250 1500 Individual Mean Mucin Concentrations Distance between arrows on same horizontal plane is significant at p=<0.03

  7. VIII. Individual and Population Variation of MUC7and MUC5B Mucinsin Repeated Collections of Stimulated Whole Saliva Two-level Nested Analysis of Variance (nANOVA)

  8. X. Individual and Population Variation of Salivary Flow Ratesin Repeated Collections of Unstimulated and Stimulated Whole Saliva Two-level Nested Analysis of Variance (nANOVA)

  9. XI. Levels of Correlation Between Individual Mean Mucin Concentrations and Mean Flow Rates Comparison r Adjusted r2 p-value • Unstimulated MUC5B and MUC7 concentrations 0.927 0.824 0.0078 • Unstimulated and stimulated MUC7 concentrations 0.604 0.213 0.20 • Unstimulated and stimulated MUC5B concentrations 0.501 0.064 0.31 • Stimulated MUC5B and MUC7 concentrations 0.695 0.354 0.13 • Unstimulated MUC5B concentration and flow rate 0.015 0.000 0.978 • Unstimulated MUC7 concentration and flow rate 0.335 0.000 0.516 • Stimulated MUC5B concentration and flow rate 0.269 0.000 0.606 • Stimulated MUC7 concentration and flow rate 0.176 0.000 0.739

  10. XII. Conclusions and Implications from the Study of Individual Variation of Mucin Concentrations in Unstimulated Whole Saliva • These studies suggest that each person within an age range of 23-31 years old has a distinctive signature mean MUC7 mucin concentration in their resting saliva. Limited individual variation indicates that these signature means range from very low to high mucin concentrations. • The coefficients of variation are similar for all unstimulated MUC7 mucin concentration means in the test group. Thus as the mean mucin concentration decreases, the absolute range of variation narrows to the point that a single collection could identify with confidence those individuals who possess very low mean mucin concentrations in their resting saliva. • There is the potential for detecting possible long-term impacts of mucin concentration on oral health if broader-based studies confirm that mucin levels in unstimulated saliva exhibit limited individual variation while still showing a wide range of population variation. • When viewed as analytes for diagnostic or forecasting purposes, the two mucins illustrate that concentrations may be the same or very different in unstimulated and stimulated salivas and that each analyte may exhibit different but characteristic levels of individual variation.

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