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KOREA_U_SEOUL

Pearl-coli: Escherichia coli converting CO 2 i nto P earl powder. KOREA_U_SEOUL. Team. 2013 iGEM KOREA_U_SEOUL. KOREA_U_SEOUL. 2009 Integrated Heavy Metal Detection System 2010 Heavy Metal Gang Captured By Capsule Cop 2011 Synthesis of Synthetic Micro-Alkanes( 'Synfuels')

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KOREA_U_SEOUL

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  1. Pearl-coli: Escherichia coli converting CO2 into Pearl powder KOREA_U_SEOUL

  2. Team 2013 iGEMKOREA_U_SEOUL

  3. KOREA_U_SEOUL 2009 Integrated Heavy Metal Detection System 2010 Heavy Metal Gang Captured By Capsule Cop 2011 Synthesis of Synthetic Micro-Alkanes('Synfuels') in Engineered Escherichia coli 2012 Rice Guardian& bio logic gate 2013 iGEMKOREA_U_SEOUL

  4. Background Design & Parts Results Application & Improvement Human Practice 2013 iGEMKOREA_U_SEOUL

  5. Background 2013 iGEMKOREA_U_SEOUL

  6. CO2 HCO3- 2H+ H+ H2O CO32- 2013 iGEMKOREA_U_SEOUL

  7. Design & Parts 2013 iGEMKOREA_U_SEOUL

  8. To design Pearl-coli producing nacrein on the cell surface. 2013 iGEMKOREA_U_SEOUL

  9. Inhibit forming CaCO3 pBAD pBAD pBAD BBa_K1146002 YfaL_NacreinDR BBa_K1146001 YfaL_NacreinCA BBa_K1146003 YfaL_Nacrein R pBAD BBa_K1146000 YfaL_Nacrein(WT) CA CA R R CA CA CA Q. Why do we use 4 parts ?? Forming CaCO3 A. Forming CaCO3 To check the function of CA and R !! Inhibit forming CaCO3 2013 iGEMKOREA_U_SEOUL Fig2. parts

  10. Results 2013 iGEMKOREA_U_SEOUL

  11. 1. Cloning 4 types of nacrein clones → pB4 vector modified pETvector for protein purification 2. Expression test & Protein purification → Check protein expression 130 95 72 NA: Nacrein DR: Deleted Repeat CA: Carbonic anhydrase R: Repeat 55 43 M NA(S) NA(I) DR(S) DR(I) CA(S) CA(I) R(S) R(I) 2013 iGEMKOREA_U_SEOUL

  12. 3. CA activity of nacrein protein Expectation CO2 + H2O → HCO3-+ H+ Carbonic Anhydrase If nacrein has carbonic anhydrase activity, ->pH will be lowered. 2013 iGEMKOREA_U_SEOUL

  13. 4. CaCO3precipitation Expectation Ca2+ + HCO3-→ CaCO3 + H+ If Nacrein inhibits CaCO3 precipitation, -> pHwill slowly drop. 2013 iGEMKOREA_U_SEOUL

  14. Application & Improvement 2013 iGEMKOREA_U_SEOUL

  15. 4 types of nacrein clones Carbonic anhydrase activity of nacrein The role of nacrein in precipitationof CaCO3 2013 iGEMKOREA_U_SEOUL

  16. The problem of tag Prokaryote host change Eukaryote 2013 iGEMKOREA_U_SEOUL

  17. Global Warming Calcium Supplement Reducing CO2

  18. 2013 iGEMKOREA_U_SEOUL

  19. Our research can be basement for making real pearl 2013 iGEMKOREA_U_SEOUL

  20. Human Practice 2013 iGEMKOREA_U_SEOUL

  21. Creative Challenger Program (CCP) Cote d'Ivoire Seoul 2013 Nolto EXPO KUAS homepage 2013 iGEMKOREA_U_SEOUL

  22. Q&A 2013 iGEMKOREA_U_SEOUL

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