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LIPOSOM

LIPOSOM. Agustina Setiawati. DEFINISI. Hydrophilic. Hydrophobic. Liposom adalah dimensi koloid berupa vesikel yang mempunyai struktur fosfolipid bilayer dan mempunyai muatan permukaan tertentu ( positif , negatif atau netral ). STRUKTUR UMUM. Classes of Liposomes. Conventiona l.

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LIPOSOM

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  1. LIPOSOM AgustinaSetiawati

  2. DEFINISI Hydrophilic Hydrophobic Liposomadalahdimensikoloidberupavesikel yang mempunyaistrukturfosfolipidbilayerdanmempunyaimuatanpermukaantertentu (positif, negatifataunetral)

  3. STRUKTUR UMUM

  4. Classes of Liposomes • Conventional Cationic • Immuno

  5. Liposome kationik Liposome anionik Liposome non-ionik Liposome Zwitterionik Which one can form complexes with DNA?

  6. Kationik Liposome Cationik liposome forms complex with DNA (negative charge) Net charge of the complexes is positive so it can be interact to cell membrane

  7. Mekanisme

  8. Mekanisme…

  9. AnionikLiposom Can’t it deliver DNA? Yess Mekanisme? DNA tidakbisamembentukkompleksdgnliposomanionik (muatannegatif-negatif) Supayabs, DNA dibuatkomplekslebihdahuludgnkation (Ca2+)

  10. Liposome dptdigunakansbgvektorterapi gen scr in vivo atau ex vivo?

  11. 1995 liposome Cystic Fibrosis • Trials using liposome-mediated CFTR gene transfer began in 1995. • Non-viral vectors have the potential to avoid some of the critical problems observed with viral vectors, such as the immune response, limited packaging capacity, and random integration5 . • Liposomes may be mildly effective, but their activity does not last. For this approach to work, researchers need to figure out how to improve delivery, make the effects more permanent and reduce the adverse side effects. • To date, only cationic liposome-based systems have been tested in clinical trials in cystic fibrosis subjects6.

  12. Kelebihan Tidakmenimbulkanresponimunologis Ukuran DNA yang dapatdibawaberagam Modifikasilebihfleksibel

  13. http://www.wellesley.edu/Biology/Courses/219/Gen_news/i3_Gene_Therapy.jpghttp://www.wellesley.edu/Biology/Courses/219/Gen_news/i3_Gene_Therapy.jpg

  14. THANK YOU

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