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How Do Antibody Arrays Work?

How Do Antibody Arrays Work?. Anti-CD Antibodies. Anti CD-Antibodies. Antibodies Are Robotically Arrayed on a Slide. Nitrocellulose coat. Glass slide. Robot deposits 10 nL of antibody 400-450 µ spot. Robot deposits 10 nL of antibody 400-450 µ spot. Anti CD Antibodies.

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How Do Antibody Arrays Work?

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  1. How Do Antibody Arrays Work?

  2. Anti-CD Antibodies Anti CD-Antibodies Antibodies Are Robotically Arrayed on a Slide Nitrocellulose coat Glass slide Robot deposits 10 nL of antibody 400-450 µ spot Robot deposits 10 nL of antibody 400-450 µ spot

  3. Anti CD Antibodies Different Leukocytes (e.g. T cells) Express Different CD Antigens Anti CD Antibodies Leukocytes are Added to the Slide(usually PBMCs and added separately from PMNs)

  4. Anti CD Antibodies Anti CD Antibodies Leukocytes Bind to CD Antibodies(Depending on the match between Ab and Epitope) Leukocytes with non-matching epitopes do not bind Leukocytes with matching antigens are captured

  5. Anti CD-X Antibodies Anti CD-Y Antibodies Leukocytes That Are Not Captured Are Washed Away About 1000 cells are captured on any one antibody spot

  6. CD Antibody Microarrays display a pattern of cell capture that distinguishes betweenhealthy and inflammatory conditions CD antibodies in the array Lymphocytes Granulocytes

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