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DNA sequence controls expression of gene involve in cancer. DAI XILEI DING MEI YEE HASSANBHAI AMMAR MANSOOR SEE HAI YUN SELENA WU MEIYUN TAN HENG LIANG. BACKGROUND. Mitochondria as the central control point of apoptosis Solange Desagher and Jean-Claude Martinou
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DNA sequence controls expression of gene involve in cancer DAI XILEI DING MEI YEE HASSANBHAI AMMAR MANSOOR SEE HAI YUN SELENA WU MEIYUN TAN HENG LIANG
BACKGROUND Mitochondria as the central control point of apoptosis Solange Desagher and Jean-Claude Martinou Trends in Cell Biology, 2000, 10:9:369-377
AIMTo understand the function of Bcl-2 genes and its association with cancer by optimally utilizing BioInformatics
RESULTS Question 1
RESULTS Question 2
RESULTS Gene sequence M13994 Protein sequence AAA51813
RESULTS Question 3
RESULTS Question 4 • Identify up and downstream of coding region • Analyse sequence to choose primer: http://www.emsembl.org • Key in coding region = forward primer ATG (start codon) sequence • 18-20 nucleotides upstream
RESULTS Question 5 • Step 1: Put sequence into DNAstar Editseq • Step 2: open sequence in MapDraw
RESULTS Protein_id=“AAA51813.1” Question 6
RESULTS Question 6
RESULTS Question 6 From another online protein databasehttp://www.expasy.org/prosite/
RESULTS Method 1 Question 7 • Go to ENSEMBL website: http://www.ensembl.org • Go to human genome, search for “Bcl-2” • Result page: This search gives the list of the same protein found in different organisms
RESULTS Method 2
RESULTS The taxonomy report of the Blast result will categorize Bcl-2 and its related proteins e.g. Bcl-xL according to different organisms.
Conclusion • With the aid of BioInformatics, example: • Databases • Search engines • Online scientific softwares • we are able to carry out our research efficiently, easily and effectively.
The End DAI XILEI DING MEI YEE HASSANBHAI AMMAR MANSOOR SEE HAI YUN SELENA WU MEIYUN TAN HENG LIANG