180 likes | 355 Views
Human Intervention in evolution. Chapter 16. Selective breeding. Artificial selection used to improve herd quality. Select best male to mate with breeding females.
E N D
Human Intervention in evolution Chapter 16
Selective breeding • Artificial selection used to improve herd quality. • Select best male to mate with breeding females. • The deliberate selection by a breeder of specific animals to provide the genetic material for the next generations-Selective breeding. • Contrast of random mating
Artificial Selection • Occurs when breeders favour particular inherited features in their livestock because of their economic value or aesthetic value & use selective breeding to enhance those features & inc. their frequency. • Contrast to Natural Selection as favours aesthetic/economic features, not those for survival/reproduction. • Not necessarily features that good for survival/reproduction • Negative feature stays in gene pool only by humans intervening.
Reproductive technologies-Human intervention Artificial insemination • Involves collecting semen from a stud animal and place it into reproductive tract of female-same species • Inc. no of offspring stud animal can make. Split sperm into 10-put into 10 diff. females. • Freezing involved. • Fertilise more females • Fertilise females that are distant • Fertilise females after dead • Specific allele will become more prominent & alternatives gone • Potential to lose valuable alleles
Oestrus synchronisation • When is possible to predict oestrus cycle of females in a narrow time frame. • Advantages: • Less time needed to test animals to see if fertile • Higher fertilisation rates & birth rates • More manageable and uniform crops of animals • Lower mortality rates of newborns
Reproductive technologies-Human Intervention Sex selection • Chromosome dyed & can see sex chromosome now. X larger than Y chromosome, more DNA so brighter. • Can separate sperm now so sex selected-males. Manipulate breeding cycle • Done through Oestrus synchronisation-become fertile in specific period. • High fertilisation rate • Lower mortality • Managing young easier-same time • Less tests needed
Reproductive technologies-Human intervention Artificial pollination • Involves: • Removal of unripe stamens • Protect stigma of selected female from stray pollen • Collection of desired pollen • Transfer of pollen to stigma • Make new species by mixing pollen & stigma of closely related species. Chemical treatment needed.
Reproductive technologies-Human intervention Cloning-No sex • Asexual reproduction-DNA from 1 parent only. • Embryo Splitting: cells of early embryo are seperated. Each ind. cell put into uterus of surrogate • Identical ind. Form • Nuclear transfer: remove nucleus from cell (enucleated). • Transfer nucleus from 1 cell into another cell. • Fuse somatic cell with enucleated cell • Downside, low success rate, constant implanations e.g. 1 in 87 eggs fertilised for CC, cloned cat, premature aging because telomere shortens. • Cloning in plants: through cuttings, runners. Cut section or parent plant.
Reproductive technologies-Human intervention Gene Transfer between species • Complete set of genes & non-coding DNA called genome. • Normal transfer of genes-parent to offspring. If unable to because of species ‘species barrier’. • Genetic engineering stops species barrier-manipulates DNA & allows it to be transferred. • Org. with foreign DNA or segment termed transgenic organism. Results in permanent change. • If cell used are prokaryote (bacteria) they’re transformed. • If cell used are eukaryotic, they’re transferred.
GMO • Genetically modified organism (GMO) refers to org. whose genetic makeup has been artificially changed. • Genotype has been modified but doesn’t involve insertion of genes from diff. species. E.g. silencing (switching off) a gene • Transferring genes done by • Micro-injection of DNA, transfer using virus, electric pulse (electroporation)
Gene Therapy • Where a faulty allele in an org. is replaced by the normally functioning allele. • Aim to treat inherited disorders • Wants to add copies of the normal allele of a gene into the cells of a target tissue, turning them on to make the protein missing in the individual.
Stem Cells • Are undifferentiated that have the ability to differentiate into many diff. and specialised cell types: • Nerve, blood, bone, heart • Replace faulty/dead cells • 3 types of stem cells: Totipotent, pluripotent, multipotent (Booklet) • Embryonic stem cells: obtained from inner cell mass of an embryo (Blastocyst). Single cell isolated from inner mass, grown, divide by mitosis & produce stem cells (pluripotent) • Adult Stem cells (somatic): from bone marrow, skin, umbilical cord. Multipotent: can give rise to blood cells.
Cloning • Reproductive cloning- produce a new organism. • Therapeutic cloning- produce stem cells for use in treatment. Done through nuclear transfer technique. Treat patient with spine injury etc… & embryo created is genetic match. • Legislation in Australia has strict controls on embryo use in labs.
Can you imagine a world with… 3 Chong Phat’s 4 DAVID’S…. F*%K!!! 4 DEMI’S!!! 2 Ha’s, Meagan’s & Duyen’s !
Genetic screening • Where DNA sample is analysed to detect the presence of 1 or more alleles associated with an inherited disorder. • Breast cancer gene • Adult screening (test if they transmit disease) • Embryo biopsy: single cell removed to see if fetus is affected • Pre-natal screening: see genetic status of fetus to see inherited disorder present • Predicitve screening: ID a perfon at risk of developing a late onset disease.
Stopping Conception • Production of a zygote & its implementation into the uterus walls called conception-any technique which prevents this called contraception. Overcoming Infertility • Infertility is the inability to conceive or carry a pregnancy to a live birth. • Pelvic inflammatory disease (PID) leads to fallopian tube problems (bacteria) • Several ways to overcome it
Overcoming Infertility Donor Insemination • Fertilise a women with donor sperm (80%). • In-vitro fertilisation (IVF) • Egg is fertilised externally with sperm (can be frozen using cryoprotectant). • Female given artificial hormones to stimulate ovaries. Eggs removed by laparoscope. Sperm added (protein coat removed)= fertilisation. Transferred to mothers uterus at 2-4 cell stage. (20%) • Pg 648
Overcoming infertility Donation of eggs • Egg donated voluntarily by another woman in IVF program. Fertilised with sperm of recipient husband. Surrogacy • Surrogate mother is a woman who agrees to have a baby for another woman. Altruistic-no money involved. • Egg is fertilised using sperm of husband of mother who is unable to have the child.