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SycE

A). YopE 1-18. SycE. E18-DHFR. DHFR. p. YopE 1-52. E52-DHFR. p. DHFR. SycE. YopE 1-53. E53-DHFR. p. DHFR. SycE. YopE 1-138. E138-DHFR. DHFR. SycE. p. YopE 1-138 -DHFR. YopE 1-52 -DHFR. YopE 1-53 -DHFR. YopE 1-18 -DHFR. B). Control. YopE 1-138 -DHFR.

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SycE

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  1. A) YopE1-18 SycE E18-DHFR DHFR p YopE1-52 E52-DHFR p DHFR SycE YopE1-53 E53-DHFR p DHFR SycE YopE1-138 E138-DHFR DHFR SycE p YopE1-138-DHFR YopE1-52-DHFR YopE1-53-DHFR YopE1-18-DHFR B) Control YopE1-138-DHFR YopE1-52-DHFR YopE1-53-DHFR YopE1-18-DHFR kDa WT yscV WT yscV WT yscV WT yscV WT yscV YopO 80 kDa 25 61 yscV yscV yscV yscV WT WT WT WT YopM 19 49 YopH S YopD 13 36 LcrV YopN YopP P 25 YopE C) YopQ 19

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