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TWIST and Senescence

TWIST and Senescence. TWIST. Transcription factor bLHL Proliferation factor Binds RUNX2 Inhibits osteoblast differentiation. Aging. TWIST differentially expressed Young fibroblasts = ↑ TWIST1 Senescent fibroblasts = ↓ TWIST1 Do senescent fibroblasts have an osteoblast-like phenotype?.

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TWIST and Senescence

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  1. TWIST and Senescence

  2. TWIST • Transcription factor • bLHL • Proliferation factor • Binds RUNX2 • Inhibits osteoblast differentiation

  3. Aging • TWIST differentially expressed • Young fibroblasts = ↑ TWIST1 • Senescent fibroblasts = ↓ TWIST1 • Do senescent fibroblasts have an osteoblast-like phenotype?

  4. Cell Types • WI-38 (lung fibroblasts) • AG11016 (skin fibroblasts) • U2Os (osteosarcoma) • BMSC (+/- differentiation factors)

  5. Gene Markers • Twist • Runx2 • Osteogenic Markers • Osteocalcin • Osteopontin • Alkaline Phospatase • Type 1 Collagen • Analysis • Real-Time PCR • Immunofluorescence

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