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This program aims to provide a scientific framework for the school-based O/L Trap Program for dengue vector control. It covers the concepts of vector control, dengue vector control, and integrated vector control and management.
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Objective • General • Provide the scientific framework of the OL Trap Program of Dengue vector control DOST School-based O/L Trap Program
Objective • Specific • Explain the concept of vector control • Explain dengue vector control • Explain Integrated Vector Control (IVC) and Integrated Vector Management (IVM) • Discuss the components of IVC from vector biology to control measures DOST School-based O/L Trap Program
Objective • Specific • Describe vector surveillance as it applies to dengue OL Trap in a classroom setting • Answer the fourteen questions enumerated in the situationer DOST School-based O/L Trap Program
Objective • Specific • Identify the following • Mosquito eggs in a lawanit paddle • Ovitrap (black tumbler and paddle) • Mosquito larva • Pupae • Positive/negative trap • Adults • Entomological forms (Ovitrap/OL Trap) • Pellets DOST School-based O/L Trap Program
Situationer • Urban / semi-urban setting • Big public elementary school in City “X” • 3-story building (~30-35 feet) • 10 classrooms per floor 16x20 feet per room) • 50-55 pupils occupy one classroom from 8am-5pm DOST School-based O/L Trap Program
Situationer • ~ 1,200-1,500 pupils • No electric fans in the classrooms • One aircon and/or electric fan at principal’s office • Houses near the school • Presence of flower vases, water containers Click Here DOST School-based O/L Trap Program
Questions • How many ovitraps/OL Traps are needed to be installed in the elementary school? • Where should the traps be installed? • When should the traps be installed? • How frequent should the traps be installed? DOST School-based O/L Trap Program
Questions • What is the procedure to be followed in installing the traps? In servicing the traps? What is the meaning of servicing the traps? • Who will install and service the traps? DOST School-based O/L Trap Program
Questions • Who will monitor and check the traps? • Monitor • Empty tumbler • Missing tumbler/paddle • Check • If positive/negative? • Is there a form to be filled-out? DOST School-based O/L Trap Program
Questions • What index needs to be obtained? • What formula is to be used? • What is the meaning of the index obtained? • Who will record and compute for the index? DOST School-based O/L Trap Program
Questions • To whom should the data be submitted to? Why? • For how long will this ovitrapping be done? DOST School-based O/L Trap Program
What’s inside? • Vector • Surveillance • Prevention and Control DOST School-based O/L Trap Program
Vector • An arthropod (insects, mites, ticks) that are capable of: acquiring, supporting the development and transmitting a pathogenic agent (bacteria, viruses, parasites) from one host to another host Example: mosquito Aedes sp. transmittingdengue virus DOST School-based O/L Trap Program
Vector Control • suppression of a target population by using measures to alter the vector reproductive capacity or potential (Vector Genetics Course, 2010, India) DOST School-based O/L Trap Program
Dengue Vector Control • Application of various anti-DEN mosquito measures to alter the life cycle of the mosquito DOST School-based O/L Trap Program
Dengue Vector Control • Specific Targets • Prevent and cut dengue transmission by: • Targeting larval stage through • Trapping • Larviciding • Container management • Environmental sanitation DOST School-based O/L Trap Program
Dengue Vector Control • Specific Targets • Prevent and cut dengue transmission by: • Targeting adult stage through • Adulticiding • Space-spraying • Aerosol treatment • Genetic manipulation of reproductive capacity of mosquito DOST School-based O/L Trap Program
Dengue Vector Control • Specific Targets • Prevent and cut dengue transmission by: • Prevention of man-mosquito contact • curtains • Screening of doors and windows • Long pants • Repellants DOST School-based O/L Trap Program
Integrated Vector Control • Combination of 2 or more vector control activities supplementary to each other towards reduction of dengue incidence DOST School-based O/L Trap Program
Integrated Vector Management • utilization of technical and managerial measures to bring about an effective degree of vector suppression to prevent and control transmission DOST School-based O/L Trap Program
Components of IVC • Vector biology/behavior • Surveillance tool (Ovitrap/OL Trap surveillance index) • Critical Threshold Density Index (effective degree of vector suppression [Ovitrap index] • Control Measures • Monitoring and evaluation DOST School-based O/L Trap Program
The Vector DOST School-based O/L Trap Program
Vector Biology DOST School-based O/L Trap Program
Morphology DOST School-based O/L Trap Program
Vector Characteristics • Biting Habits • “daybiters” with two peaks of biting time: • 1 to 2 hours after sunrise • 1 to 2 hours before sunset • only female mosquitoes bite because of the requirement of blood for oviposition • males do not bite but feed on plant juices and nectar of flowers • man is the preferred host but in his absence, lower vertebrates serve as substitute DOST School-based O/L Trap Program
Vector Characteristics • Flying Habit • do not fly great distances • farthest flight distance is within 200 to 400 meters radius from their breeding places • Adults found around 50 meters from the breeding sources DOST School-based O/L Trap Program
Bionomics • at temperatures <10oC and >40oC, no developmental process takes place • females mates with male only once • Blood meal takes place after 48 hours from emergence and after mating for egg production DOST School-based O/L Trap Program
Bionomics • oviposition takes place about 2 to 5 days from blood engorgement • prefers dark-colored background for oviposition • lays egg 3 to 4 times in its lifetime • 60 to 100 eggs per batch • Survival potential: 20-30 days DOST School-based O/L Trap Program
Life Cycle (10-12 days) Adult -Maxillary pulps shorter than probosis -Wings uniformly grayish black -Body and legs are black with distinctive white patches throughout -Thorax has markings Eggs: - Black and oval in shape - Laid singly above water surfaces of containers - Without float - Viability: 6 months to 1 year due to the presence of chorion 2 - 3 days 1 - 2 days Larva: - Feeding stage - Breeds in clean and non-polluted water - Short and stout siphon with one pair of hair tuft - Rests at an angle to the water surface Pupa -Non-feeding stage -Breeding trumpet is long, slender with narrow opening 6 - 8 days DOST School-based O/L Trap Program
Surveillance DOST School-based O/L Trap Program
Vector Surveillance • Collection and interpretation of data which can be used to guide mosquito vector control operations • Establish baseline • Presence or absence of vector • Species? • Density? • Changes? DOST School-based O/L Trap Program
Dengue Surveillance Tool • Classical OVITRAP/LARVITRAP (black tumbler, paddle, water) • Modified OVITRAP is the OLTRAP (black container, paddle, water and organic larvicide) • 2 adults (females) emerging per day (Malaysian experience) DOST School-based O/L Trap Program
Difference between tools DOST School-based O/L Trap Program
Ovitrap Index Total Number of positive traps Total Number of traps planted If OI is >10%, the place is vulnerable to dengue transmission. X 100% DOST School-based O/L Trap Program
Critical Threshold Density Index (CTDI) • Actual target for the degree of vector suppression • Predictive value for the prevention of dengue transmission DOST School-based O/L Trap Program
Remember: • A positive trap means there is at least one mosquito laying its eggs in the sampling site (surveillance site) • Aim is eliminate this one mosquito laying its eggs DOST School-based O/L Trap Program
What is DOST Mosquito Ovi/Larvicidal (OL) Trap System? • Is a system used to control the population of the • dengue carrying Aedes mosquitoes. • It has three important parts: a black container, a • small strip of lawanit measuring 1” x 6.5” for • mosquitoes to lay their eggs on, and pellets as • larvicide. • The OL-Trap’s ovicidal and larvicidal effect • prevent the next generation of mosquitoes from • reaching adulthood, thus curbing the Aedes • mosquito population. Tumbler Paddle Pellets DOST School-based O/L Trap Program
HOW THE OL-TRAP WORKS? • The idea is to attract mosquitoes particularly the Aedesaegypti and albopictus (dengue carrier) to lay their eggs on the lawanit paddle. • The trap creates optimum conditions for this species to lay its eggs: stagnant, clean, clear water, dark area and damp rough surface. DOST School-based O/L Trap Program
The eggs will either be trapped in the lawanit paddle or find its way into organic solution, where they are killed before transforming into adults. Eggs Larvae DOST School-based O/L Trap Program
Distribution of OL Trap DOST School-based O/L Trap Program
Distribution of OL Trap CLASSROOM 1 OL Trap –indoor and 1 OL Trap-outdoor CLASSROOM 1 OL Trap –indoor and 1 OL Trap-outdoor CLASSROOM 1 OL Trap –indoor and 1 OL Trap-outdoor CLASSROOM 1 OL Trap –indoor and 1 OL Trap-outdoor SCHOOL CLASSROOM 1 OL Trap –indoor and 1 OL Trap-outdoor
Distribution of OL Trap 1 OL Trap indoor 1 Tumbler (Black Container) 1 sachet of pellet per week 1 pc paddle per month 1 OL Trap outdoor 1 Tumbler (Black Container) 1 sachet of pellet per week 1 pc paddle per month CLASSROOM
Servicing the OL Trap • OVITRAPS are installed and serviced every seven days in locations where transmission of dengue is probable • nursery schools • pre-schools • elementary schools • Hospitals • community with confirmed dengue cases • OvitrapIndex = > 10% DOST School-based O/L Trap Program
Before OL Trap Installation • Clean-up all possible breeding sites/containers found inside/outside the classrooms – DO SEARCH and DESTROY Artificial containers (man-made) Natural containers 1. Tree Holes, bamboo stumps 2. Leaf axils, fallen leaves 3. Ground Depressions 1. Flower vases and pot plates 2. Pails, water-storage jars, basins 3. Discarded receptacles 4. Choked roof gutters 5. Gully Traps 6. Unused toilet bowls and cisterns 7. Aircon Tray and dish rack tray 8. Concrete drains DOST School-based O/L Trap Program
Where to set up the DOST OL-Trap? A. Indoor: • Under the sink • Corner of the room • Under the cabinet • Under the bed • Inside the comfort room B. Outdoor (covered area): • Corner of the classroom • Near vegetation • Piles of woods/hollow blocks • Garden DOST School-based O/L Trap Program
Setting the OL Trap • Step 1 • Fill the plastic tumbler with tap water up to the lined mark just above the DOST logo (~250 ml) DOST School-based O/L Trap Program
Setting the OL Trap • Step 2 • Pour one pack OL pellets into the plastic cup • Stir thoroughly to ensure pellets are dissolved DOST School-based O/L Trap Program
Setting the OL Trap • Step 3 • Place the lawanit stick in the solution with the rough surface facing up DOST School-based O/L Trap Program
Setting the OL Trap • Step 4 • Place 1 cup each inside and outside the classroom in a low-lying and dark area • If outside, protect from direct sunlight and rainfall • Take care not to spill the contents • Keep out of reach of children and animals DOST School-based O/L Trap Program