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What do you think?. 1. What do the muscles in your body use when you run? 2. What thing goes through every part of your body? 3. Where are the instructions to what you are kept?. http://www.mynamesnotmommy.com/wp-content/uploads/2013/05/question-mark.png. How can we see these?.
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What do you think? 1. What do the muscles in your body use when you run? 2. What thinggoes through every part of your body? 3. Where are the instructions to what you are kept? http://www.mynamesnotmommy.com/wp-content/uploads/2013/05/question-mark.png
How can we see these? • Mitochondria in a cell 2. Red Blood cells 3. Nucleus of a cell
Microscopy - an introduction • Microscopes are instruments designed to produce magnified visual or photographic images of small objects. The microscope must accomplish three tasks 1. produce a magnified image of the specimen • separate the details in the image, • render the details visible to the human eye or camera.
Microscope One or more lenses that make an enlarged image of an object.
Simple Microscope • Light passes through only 1 lens. • Example: magnifying glass
Compound Microscope • Lets light pass through an object and then through two or more lenses.
Stereoscopic Microscope • Gives a three dimensional view of an object. (Examples: insects and leaves) • Used for dissections
Electron microscopes – use a beam of electrons instead of a beam of light to magnify the image
Electron Microscopes • can achieve 3D images using electrons
The Scanning Electron Microscope • produces a 3-dimensional image of specimen’s surface features spider head of a butterfly
Scanning electron microscopy (SEM) Types of specimens: -Whole organisms -Natural tissue surfaces -Exposed tissue structure What is this? A flea magnified 50, 000 X
Longitudinal section of cilium Cross section of cilium 1 µm Transmissionelectronmicroscope (TEM) • Provides for detailed study of the internalultrastructure of cells • a beam of electrons is transmitted through the specimen for a 2D view Figure 6.4 (b) cilia on rabbit lungs
Transmission electron microscope Chloroplast from a tobacco leaf H1N1 virus
What is the difference between a… VIRUS and CELL? E.coli bacterial cells
VIRUS BACTERIA • can’t live on its own- must - can exist on its own live inside another cell • much smaller (20 – 400nm) - larger (1000 nm = 1μm) • none are beneficial - some can be beneficial (bacteria in gut) • no cell wall, only a protein - outer cell wall coat - cannot be killed by antibiotics - are killed by antibiotics
Modified from http://cochrane.rockyview.ab.ca/Members/lynnmmoore/science-10/unit-3-cycling-of-matter-in-living-systems /ch-7-the-basis-of-life/s10-lesson-1-cells-and-cell-theory/MICROSOPE%20TYPES%20PPT-1.2ppt.ppt/view