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Oxidative Burst

Oxidative Burst. Doug Tischer. General Idea. Galactose oxidase. State 1. No signal. Catalase. Buffers the cell from any leaky GOase expression. State 2. Galactose oxidase. Signal. = H 2 O 2 = H 2 O = O 2. Catalase. (Bad cell). System Design. luxR. GOase. tetR. LuxR. katE+ssrA.

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Oxidative Burst

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  1. Oxidative Burst Doug Tischer

  2. General Idea Galactoseoxidase State 1 No signal Catalase Buffers the cell from any leaky GOase expression State 2 Galactoseoxidase Signal = H2O2 = H2O = O2 Catalase (Bad cell)

  3. System Design luxR GOase tetR LuxR katE+ssrA pTET

  4. System Response 100% Relative Concentration 0% 120 30 90 0 60 150 Time (min) Stimulus (AHL) KatE degradation lags behind GOase production GOase TetR KatE H2O2

  5. Parts Breakdown luxR GOase tetR lacI + pL lux pR B0034 F2622 Q04400 From Arnold lab katE+ssrA B0034 B0015 Q04400 pTET Genomic PCR BBa_M0050

  6. Status

  7. Connection to other projects • Source of galactose could be from lactose breakdown. • Cells could be triggered to adopt this fate from SSM. • ROS species could be detrimental to vitamin synthesis (among many other things). • Vitamins could be harmful to ROS generation if they are antioxidants.

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