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Rodent Surgery Course

Rodent Surgery Course. Why????. Laws and regulations dictate appropriate procedures and care. Laws and Regulations. You have a contract with the AIC Surgical variables can affect animal welfare and research. You Have a Responsibility to the Animal. Success:. Requires careful planning.

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Rodent Surgery Course

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  1. Rodent Surgery Course

  2. Why???? • Laws and regulations dictate appropriate procedures and care

  3. Laws and Regulations

  4. You have a contract with the AIC • Surgical variables can affect animal welfare and research

  5. You Have a Responsibility to the Animal

  6. Success: • Requires careful planning

  7. Challenges…. • Same person is surgeon, anesthetist, scrub nurse • Experimental design requires repetitive surgeries • Small body size of rodents mandates use of microsurgery or ophthalmic instruments • Immunodeficient rodents

  8. Major Survival Surgery • “Any surgical intervention that penetrates and exposes a body cavity or any procedure which produces permanent impairment of physical or physiologic functions”

  9. Minor Survival Surgery • Surgery that “does not expose a body cavity and causes little or no physical impairment”

  10. Non rodents: Dedicated surgical facility

  11. Rodents: Surgical facility not required

  12. Rodent Surgery Area • Room or part of a room that is easily sanitized and not used for other activities when rodent surgery is in progress

  13. Rodent Surgery Area • Subdivided into: • Place for cages of rodents awaiting surgery • Prep area • Surgery area • Recovery area

  14. Anesthesia • Inhalation • Injectable

  15. Instruments • Quality • Appropriate size for procedure • Correct utilization

  16. Surgical Pack • Order • Sterilization indicators

  17. Wound Closure

  18. Surgical Needles

  19. Surgical Adhesive

  20. Preoperative Management • Animal health status • Period of stabilization • Proper handling and restraint • Stress-reduction • Food and water NOT withheld • AVOID antimicrobial agents

  21. Perioperative Management • Prepare surgery area • organize • disinfect

  22. Sterilization of Instruments • Autoclave

  23. Indications of Sterilization

  24. Glass Bead Sterilizer • Provides rapid sterilization

  25. Chemical • Time lengthy • Kill spores?????

  26. Patient Prep

  27. Prevent Hypothermia

  28. Surgeon Prep

  29. Operative Technique • Follow aseptic surgery procedures • Utilize good surgical technique • Tunnel vascular catheters

  30. Operative Care • Monitoring the patient

  31. Post-operative Care • Analgesia • Hydration • Nutrition • Wound care • Suture removal

  32. Maintaining Records • Procedure and date • Post op care • Suture removal

  33. Monitoring Outcomes

  34. PI’s for donating extra animals for this lab!!

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