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Factors affecting on semen quality

Factors affecting on semen quality. ผศ.น.สพ.ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธ ุ์. ให้นักศึกษาอธิบายปัจจัยที่ทำให้เชื้ออสุจิตายมา 1 ตัวอย่าง. References.

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Factors affecting on semen quality

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  1. Factors affecting on semen quality ผศ.น.สพ.ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธุ์

  2. ให้นักศึกษาอธิบายปัจจัยที่ทำให้เชื้ออสุจิตายมา 1 ตัวอย่าง

  3. References • Barth A.D and Waldner C.L. 2002. Factors affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine. Can Vet J 43(3):274-284 • Hafez.E.S.E and Hafez.B. 2000. Reproduction in farm animals 7 th edition. Lippincott Williams & Wilkins. 509pp • http://vet.kku.ac.th/chaiwat/teaching/teaching2010

  4. Objectives • บอกสาเหตุที่มีผลกระทบต่อเชื้ออสุจิได้ • สามารถอธิบายปัจจัยจากตัวสัตว์ต่อคุณน้ำภาพของเชื้อ • สามารถชี้ให้เห็นถึงอิทธิพลของสิ่งแวดล้อมที่มีผลต่อคุณภาพน้ำเชื้อ • สามารถบอกถึงปัจจัยที่อาจมีผลต่อคุณภาพน้ำเชื้อในขั้นตอนการผสมเทียม

  5. Breeding soundness evaluation Pedigree Progeny test - Long time - More costly

  6. Breeding soundness evaluation Breeding Index (USA TPI=Type production index) (Canada LPI= Lifetime profit index) (New Zealand BV’S = Breeding values) (Japan NTP=Nippon total index)

  7. Terminology of semen • PTA (ETA) : Predicted or estimated transmitting ability • EBV : Estimated breeding value (Canada) • PTAT : Predicted transmitting ability of type traits • Reliability

  8. Factors on Semen quality • Bulls effect • Feeding • Diseases • Semen collection management • Environmental effects

  9. Bulls affecting • Age • Scrotal circumference • Breeds • Body condition score • Physical abnormalities • Frostbite ???

  10. Effect of Age

  11. Effect of Breeds

  12. Effect of BCS

  13. Effect of Physical abnormalities

  14. Effect of Scrotal circumference Minimum Recommended Scrotal Circumference inCentimeters by Age for Bulls Age (months)Scrotal Circumference (cm) 15 30 >15-18 31 >18-21 32 >21-24 33 >24 34

  15. Effect of scrotal frostbite

  16. Feeding on semen quality - Puberty --> scrotal circumference - semen collection time - Bull > 6 years - Boar > 3 years - Stallion > 20 years

  17. Feeding NRC (National Research Council) Young animals - Low energy --> delay semen production - Protein -->less affecting - High energy ---> low libido

  18. Thailand --> Late puberty - roughage - climates

  19. Low feeding - Testosterone --> decrease - low libido - Obesity --> low libido and sexual activity

  20. Vitamins Vitamin A Pituitary gonadotropins Testicular degeneration Vitamin E ---> Male organ

  21. Minerals - Iodine --> poor libido - Copper - Cobalt - Zinc - Manganese

  22. Toxins - High gossipol --> epididymal cell - radiation --> spermatogenesis - estrogen feed ---> failure of sperm transport in the female tract

  23. Diseases - Virus (IBR, BVD, FMD) - Bacteria (Brucellosis) - Parasite (Blood parasites)

  24. Semen collection management - Training - Frequency 1-2 per week - hoof problem --> electro-ejaculator

  25. Environmental effects 1. Temperature - Motility - Temperature shock - Heat shock - Cold shock 2. Photoperiod & season Long time --> Longevity sperm 3. Oxygen ion O2 ---> H2O2

  26. 4. Acidity pH 6.7- 6.9 Sperm metabolism --> Lactic acids ---> Buffers 5. Osmostic pressure Permeabiliity 6. Metal Lead, Mercury 7. Bacteria 8. Detergent & Toxin

  27. Risk of environment factors 1. Semen collection 2. Storage and freezing 3. Storage and transportation 4. Thawing and insemination

  28. Semen collection Bulls prepare - Shower - Washprepuce with NSS 0.9 % Material prepare - Detergent - Sterile Collection time - Light - Temperature

  29. Storage and freezing 1. Freezing 37 C 5 C -20 C - 79C -196C

  30. 2. Extender - protection cold shock and cryo-protectant - Energy source - Buffering - Osmotic pressure - Bacteria inhibition

  31. Extender Egg-Yolk Tris - Tris ---> buffers - citric acid --> buffers - Glucose --> energy, osmotic pressure - Penicillin, Streptomycin, Polymixin B - Glycerol --> cryo- protectant - Egg-yolk --> cyro-protectant

  32. Storage and Transport Semen bank tank - coding - liquid nitrogen AI center tank - liquid nitrogen--> 2 weeks Inseminator tank - liquid nitrogen --> day by day

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