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Electrotaxis of lung cancer cells in a multiple-electric-field chip

Electrotaxis of lung cancer cells in a multiple-electric-field chip. Speaker: Ching Wen Huang Adviser: Ji Yen Cheng 2009.01.21. Historical Background. The common origins of Electrophysiology and Bioelectricity. Leydon jar. ( 徐在新 , 從法拉第到麥克斯韋 , 凡異出版社 )

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Electrotaxis of lung cancer cells in a multiple-electric-field chip

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  1. Electrotaxis of lung cancer cells in a multiple-electric-field chip Speaker: Ching Wen Huang Adviser: Ji Yen Cheng 2009.01.21

  2. Historical Background The common origins of Electrophysiology and Bioelectricity Leydon jar (徐在新, 從法拉第到麥克斯韋, 凡異出版社) (C.D. MaCaig, Physiol Rev., vol. 85, July 2005) <1>

  3. Electrotaxis (galvanotaxis): Movement of organisms or cells in response to an electric field. Power supply - + Physiological Electric Field (EF): About 1~100mV/mm, existing in wound, embryo’s skin, interface between tumor and normal tissue, etc. (C.D. MaCaig, Physiol Rev., vol. 85, July 2005) <2>

  4. Device for electrotaxis study(McCaig et al.) • Design features: • Defined geometry of culture chamber • Agar-gelled salt bridges • Shortcomings: • large chamber size • not well-sealed • bulky (B. Song, Nature Protocols, vol. 2(6), 2007) <3>

  5. Our electrotactic chip • Advantages: • well-sealed • negligible Joule heating • Multiple EF strengths EF strength = I σ*W*h <4>

  6. Our Electrotactic Device <5>

  7. Cell Migration under EF Lung cancer cells: CL1-0 – weak metastasis / no response CL1-5 – high metastasis / move toward the anode - + EF CL1-0 210mV/mm 340mV/mm 0mV/mm 75mV/mm CL1-5 <7>

  8. Response 2: Cell Orientation under EF Under the applied EF, obvious orientation of CL1-5perpendicular to the direction of EF is shown. before treating treating 7hr - + EF <8>

  9. Possible Mechanism A physiological EF physically moves charged receptor molecules exposed on the outer surface of the lipid bilayer and creates receptor asymmetry between cathodal- and anodal-facing membranes. EGFR on corneal epithelial cells <10>

  10. Conclusion & Future Work • A well-sealed electrotactic chip which can provide multiple EF strengths was designed and fabricated. • Positive correlation between metastasis and electrotaxis of lung cancer cells was observed. • The orientation of CL1-5 was observed following the migration, implying different signal pathway triggered by physiological EF • Surface proteins which are related to metastasis of lung cancer will be studied under physiological EF. <11>

  11. Thanks for your attention

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