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Molecular genetics of gene expression

Molecular genetics of gene expression. Mat Halter and Neal Stewart 2014. Discussion questions. What are the differences between DNA and RNA? Describe the main parts of a gene and its functions. What role do cis-regulatory elements and trans-acting factors play in gene regulation?

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Molecular genetics of gene expression

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  1. Molecular genetics of gene expression Mat Halter and Neal Stewart 2014

  2. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and its functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  3. http://www.yellowtang.org/cells.php

  4. A Chromosome is composed of nucleosome-bound DNA called chromatin

  5. The nucleosome Fig F09-30A. Nucleosome structure. (Lodish et al., 2000).

  6. Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid April 25, 1953 Francis Crick James Watson Figure 6.2 http://www.stern.de/_content/50/44/504453/watson_crick_500.jpg

  7. Nucleotide base pairing A’s pair with T’s G’s pair with C’s Nucleotide base pairing occurs through “hydrogen bonding” Strands have directionality from 5’ to 3’ and when paired strands are in “antiparallel” orientation

  8. DNA and RNA are structurally similar

  9. Some differences of RNA and DNA

  10. Difference between thymine and uracil

  11. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  12. The Central Dogma Transcription DNA RNA Protein

  13. Eukaryotic gene structure Figure 6.5

  14. TF TF TF TF TF RNA polymerase II AAAAA Transcription of DNA into mRNA mRNA = messenger RNA

  15. Eukaryotic genes contain introns which are spliced to form mature mRNA

  16. mRNA structure differs between prokaryotes and eukaryotes Polycistronic prokaryotic message Monocistronic eukaryotic message

  17. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  18. Cis- acting regulatory elements • Promoter region- Located immediately upstream of the transcription start site and serves as a binding site for the RNA -polymerase II complex. • Enhancer region- Promotes transcription by recruiting histone-modifying enzymes that open the chromatin structure, making the sequence more accessible—sometimes acts in trans. • Insulators- Sequences that have the ability to protect genes from inappropriate signals emanating from their surrounding genome. Vascular Tissue-Specific Promoter GUS gene Terminator http://www.forschenistkunst.at/bildbewertung/objekt35.php?id=35

  19. Transcription factors: proteins that bind to promoters to affect transcription • Transcriptional activators- Recruits the RNA polymerase complex to the transcription start site by binding to either sequences in the promoter or distant cis-acting elements to increase transcription. • Transcriptional repressor- Prevents transcription of a gene by interfering with RNA polymerase activity on a promoter Promoter LacZ LacY LacA RNA Polymerase mRNA VIDEO

  20. Transcription of DNA into mRNA TF TF TF TF TF RNA polymerase II AAAAA mRNA = messenger RNA The TFs in this case are transcriptional activators

  21. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  22. The Central Dogma of Molecular Biology Translation DNA RNA Protein

  23. http://www.yellowtang.org/cells.php

  24. The central dogma revisited • The order of the DNA template or coding strand is 3’ to 5’ • This determines the order of the mRNA strand (5’ to 3’) because DNA template is complementary to the mRNA strand.

  25. Transfer RNA (tRNA) Amino Acid

  26. AUG UCG ACA GGC UGA

  27. Initiation of translation

  28. Assembly of the small ribosomal subunit and initiator tRNA onto the mRNA

  29. The start codon is found by scanning downstream from the 5’ end of the mRNA VIDEO

  30. Amino acid backbone

  31. 20 amino acids

  32. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  33. Gene expression regulation • Transcriptional level: • Transcription factors (environmental, physiological) • Alternate splicing • Translational level: • Elongation factors • RNA interference

  34. Discussion questions • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different ways can gene expression be regulated?

  35. http://www.youtube.com/watch?v=GkdRdik73kU • http://www.youtube.com/watch?v=GkdRdik73kU

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