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CD3 + HLA-DR +

6. P=ns. P=ns. 5. 4. 3. 2. 1. 0. 0. 10. 20. 30. 40. 50. Early rejectors. Non rejectors. A. B. C. R=-0.21 P= 0.06. P=ns. CD3 + CD25 high. CD3 + CD25 high. CD3 + CD25 high. CD3 + HLA-DR +. CD3 + HLA-DR +. CD3 + HLA-DR +. D. E. F. 60. R=-0.48 P=0.0001. R=-0.45

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CD3 + HLA-DR +

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  1. 6 P=ns P=ns 5 4 3 2 1 0 0 10 20 30 40 50 Early rejectors Non rejectors A B C R=-0.21 P= 0.06 P=ns CD3+CD25high CD3+CD25high CD3+CD25high CD3+HLA-DR+ CD3+HLA-DR+ CD3+HLA-DR+ D E F 60 R=-0.48 P=0.0001 R=-0.45 P=0.0001 R=-0.53 P=0.019 P=ns 50 40 CD3+CD25low CD3+CD25low CD3+CD25low 30 20 10 0 0 10 20 30 40 50 CD3+HLA-DR+ CD3+HLA-DR+ CD3+HLA-DR+ Supplemental Digital Content 2- Figure 2

  2. Correlation among pre-transplant CD3+CD25high/CD3+HLA-DR+ and CD3+CD25low/CD3+HLA-DR+ ratios. Pre-transplant blood samples were triple-labelled with anti-CD25PE, anti-HLA-DRFITC, and anti-CD3PerCP and analysed by FACS (Material and Methods). Values represent the measured percentages of CD25high, CD25low, and HLA-DR+cellson CD3+ cells. Shown are the results for the entire population of patients (A and D), plus the segregated results for the groups of rejecting (B and E) and non-rejecting (C and F) patients, where each dot corresponds to a single patient. The regression line between variables is shown. Pearson correlation coefficients (R) and (P) are indicated only when statistically significant. Center lines in B, C, E and F indicate the arbitrary threshold at 0.7% of CD3+ cells.

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