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CSF: Current DIAGNOSTIC in Cuba

CSF: Current DIAGNOSTIC in Cuba. Boca Chica, RD. Marzo/2004. Familia Flaviviridae. Género Pestivirus. PPC. BD. BVD. Host. Proteins. HOMOLOGY: 85 %. Genome. HOMOLOGY: 66 - 74. %. Antibodies. CROSS SEROLOGY. ¿ What is internationally done?.

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CSF: Current DIAGNOSTIC in Cuba

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  1. CSF: Current DIAGNOSTIC in Cuba Boca Chica, RD. Marzo/2004

  2. Familia Flaviviridae Género Pestivirus PPC BD BVD Host Proteins HOMOLOGY: 85 % Genome HOMOLOGY: 66 - 74 % Antibodies CROSS SEROLOGY

  3. ¿What is internationally done? • EUROPE: ELISA Ab, ELISA Ag, DIF, Virus Isolation, PCR, sequencing (Germany) • AMERICA: DIF, ELISA Ab, sequencing (Cuba, USA, Mexico) • ASIA: DIF, NPLA, PCR, ELISA Ag, sequencing (Korean Republic).

  4. Clínico-Epizootiológico DIAGNÓSTICO VIROLÓGICO Anatomopatológico Bacteriológico National Program for CSF

  5. DIAGNOSTIC PROCEDURESIdentification of the virus and/or its components

  6. DIAGNOSTIC PROCEDURESAntibodies identification

  7. CURRENT LABORATORY DIAGNOSTIC IN CUBA : • Samples (T,S,LN) from animals with more than 30 days of vaccination. • Freezing section of tonsils, spleen, lymphatic nodes • DIF with national polyclonal conjugate DIAGNOSTIC REPORT …. In 72 hours • Maceration of organs pool (T,S and LN) • Inoculation in PK-15 or SK-6 cells/Virus isolation • Virus identification: DIF or DIP/TC, RT-PCR supernatant/diagnostic or sequencing • Genotyping DIAGNOSTIC REPORT ….. In 30 days

  8. Samples processed in 2003 by DIF Per productive sectors TOTAL= 221/196

  9. Samples processed in 2003 for viral isolation Per productive sectors TOTAL= 16/9+

  10. Composed by : • Head of the CSF Program (IMV) • Specialists from IMV (Surveillance) • Specialists from CENEDI (Pathology, Epidemiology) • Specialists from CENSA (Virology) • IMV Specialists in swine (Epidemiology) • Havana provinces • Veterinary Chief from GRUPOR, • From Genetic or other main producers as it is the case National Comission for the diagnostic of CSF Analysis of the lab results for FOCI declaration (actually ….. 15 days)

  11. DATA TREATMENT All the outcomes are the objective to be transmit to the “Sistema de vigilancia epidemiológica molecular de la PPC” for further analysis

  12. QUALITY ASSURANCE • Internal Validation of reactive • PNO • RPNO • Internal Auditoria • External Auditoria • GLP

  13. Mutation rates

  14. CURRENT CRITICAL POINTS IN DIAGNOSTIC Differential diagnostic from other related diseases Differential diagnostic from the vaccinal virus MAbs Cj for routine diagnostic ELISA’s commercially To continue the genotyping of isolates Diagnostic procedures for carriers Cryostat

  15. Immediate Outlooks • Training of specialist from CENEDI at CENSA to assume the primary diagnostic by DIF in freezing sections • Accreditation of the virology lab at CENSA as a Regional Reference Laboratory • To assign CENEDI the necessary resources for the diagnosis

  16. For a Caribbean CSF free in 2008 M A N Y T H A N K S....!!

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