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BACTERIALBASICS

Learn about the basics of bacteria, including their structure, DNA transfer processes, and adhesion zones. Explore prokaryotes, cell walls, asexual reproduction, bacterial transformation, and the role of calcium chloride solution and heat shock.

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BACTERIALBASICS

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  1. BACTERIALBASICS

  2. Bacteria Structure • Prokaryotes • No nucleus • Cell wall • DNA plasmid (ring-shaped) • Use asexual reproduction http://en.wikipedia.org/wiki/Bacteria

  3. Different Forms of DNA Transfer

  4. Bacterial Transformation • 1928-Frederick Griffith, 1944-Avery, McCarty, Macleod • Process of taking up DNA from media • DNA can be from cells from same or different species • DNA will integrate into the recipient’s chromosome http://en.wikipedia.org/wiki/Bacterial_transformation

  5. Bacterial Adhesion Zones • Adhesion zones develop in the bacteria cell membrane, although DNA cannot fit through

  6. Calcium Chloride Solution • The addition of calcium ions in CaCl2 solution shields the negatively charged phosphate groups so that the DNA can pass through the membrane when heat shocked. Heat Shock 42ºC

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