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Lab2 L actate Dehydrogenase

Lab2 L actate Dehydrogenase. Daheeya AlEnazi. Quantitative determination of lactate dehydrogenase LDH SPINREACT KIT. Why do we use it?. LDH is an enzyme that helps the process of turning sugar into energy for your cells to use.

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Lab2 L actate Dehydrogenase

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  1. Lab2Lactate Dehydrogenase DaheeyaAlEnazi Quantitative determination of lactate dehydrogenase LDH SPINREACT KIT

  2. Why do we use it? • LDH is an enzyme that helps the process of turning sugar into energy for your cells to use. • it is present in many kinds of organs and tissues throughout the body, including the liver, heart, pancreas, kidneys, skeletal muscles, brain, and blood cells.

  3. Types of LDH Isoenzymes

  4. principle LDH catalysesthe reduction of pyruvate by NADH: Pyruvate+NADH+ H+ LDH L-lactate+ NAD+ The rate of decreasein concentration of NADPH, measured photometircally, is proportional to the catalytic concentration of LDH present in sample.

  5. Clinical significance When illness or injury damages your cells, LDH may be released into the bloodstream, causing the level of LDH in your blood to rise. • High LDH: Liver disease, heart (MI), kidney diseae, skeletal muscle(musclur dystrophy) and erythrocytes(Anaemia) • Abnormally low LDH levels occur only rarely and usually aren’t harmful. • Clinical diagnosis should not be made on single test result.

  6. Procedure • Mix, incubate for 1 minute. • Read initial absorbance (A) of the sample at 340nm, start the stopwatch • And read absorbances at 1min intervals thereafter for 3 min. • Calculate the difference between absorbancesand the average absorbance differences per minute (∆ A/min)

  7. Calculation ∆ A/min x 4925 = LDH U/L • Normal Range: 120-240 U/L

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