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Quantitative Determination of Lactate Dehydrogenase (LDH) - Why Do We Use It?

This article explores the significance, types, and clinical relevance of Lactate Dehydrogenase (LDH) enzyme. LDH plays a crucial role in converting sugar into energy for cell function and is found in various organs and tissues. Discover the principle behind LDH isoenzymes and its measurement using a SPINREACT KIT. Learn about the clinical significance of LDH levels in conditions such as liver disease, heart attack, kidney issues, and more. Understand the procedure for LDH determination, including mixing, incubation, and calculating LDH units per liter. Normal LDH range and the importance of not relying on a single test result for clinical diagnosis are also discussed.

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Quantitative Determination of Lactate Dehydrogenase (LDH) - Why Do We Use It?

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  1. Lab2Lactate Dehydrogenase DaheeyaAlEnazi Quantitative determination of lactate dehydrogenase LDH SPINREACT KIT

  2. Why do we use it? • LDH is an enzyme that helps the process of turning sugar into energy for your cells to use. • it is present in many kinds of organs and tissues throughout the body, including the liver, heart, pancreas, kidneys, skeletal muscles, brain, and blood cells.

  3. Types of LDH Isoenzymes

  4. principle LDH catalysesthe reduction of pyruvate by NADH: Pyruvate+NADH+ H+ LDH L-lactate+ NAD+ The rate of decreasein concentration of NADPH, measured photometircally, is proportional to the catalytic concentration of LDH present in sample.

  5. Clinical significance When illness or injury damages your cells, LDH may be released into the bloodstream, causing the level of LDH in your blood to rise. • High LDH: Liver disease, heart (MI), kidney diseae, skeletal muscle(musclur dystrophy) and erythrocytes(Anaemia) • Abnormally low LDH levels occur only rarely and usually aren’t harmful. • Clinical diagnosis should not be made on single test result.

  6. Procedure • Mix, incubate for 1 minute. • Read initial absorbance (A) of the sample at 340nm, start the stopwatch • And read absorbances at 1min intervals thereafter for 3 min. • Calculate the difference between absorbancesand the average absorbance differences per minute (∆ A/min)

  7. Calculation ∆ A/min x 4925 = LDH U/L • Normal Range: 120-240 U/L

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