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Prostate: Transverse section. Radical Prostatectomy. Bladder neck. Apex. Radical Prostatectomy. Base/ Bladder Neck. Apex. Posterior quadrants of alternate slices have been removed and frozen after inking the surface. Lateral aspect.
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Radical Prostatectomy Bladder neck Apex
Radical Prostatectomy Base/ Bladder Neck Apex Posterior quadrants of alternate slices have been removed and frozen after inking the surface
Lateral aspect Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data Seminal Vesicle Permanent section Permanent section right left 1 7 2 6 3 5 4 5R 5 5R 5L 5L Fresh, non-frozen tissue for flow sorting Frozen section Unfixed tissue for RNA & DNA left right 4R Frozen section 4 4R 4L 4L Permanent section left right permanent section 3R 3 3R 5L 3L = Prostate carcinoma
Human prostate (lateral view) Obtaining samples of non-tumortissue from a radical prostatectomy specimen. Seminal Vesicle Permanent section Fixed tissue: In-situ hybridization right 1 left 7 2 6 3 5 4 5R 5 5R 5L 5L Fresh, non-frozen tissue: cell sorting This block is snap frozen (isopentane in liquid N2), within < 20 minutes of devitalization Frozen tissue: Immunostains Laser capture right left 4R 4 4R 4L 4L Frozen section Permanent section left right Permanent section 3R 3 3R 5L 3L = Prostate carcinoma
Normal prostate parenchyma (high magnification) Basal epithelial cells Secretory (luminal) epithelial cells Fibromuscular stromal cells
Normal prostate parenchyma (low magnification) fibromuscular stroma normal glands lymphocytes