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Need for a Public Doubled Haploid Facility ?

Need for a Public Doubled Haploid Facility ?. Thomas Lübberstedt, Candy Gardner, Mike Blanco, Uschi Frei, Elisabeth Bovenmyer. Structure. DH Production in Maize: How ? Why (Doubled) Haploids ? Who uses DHs ? Need for a Public DH Facility ?. Technologies. Haploid production

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Need for a Public Doubled Haploid Facility ?

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  1. Need for a Public Doubled Haploid Facility ? Thomas Lübberstedt, Candy Gardner, Mike Blanco, Uschi Frei, Elisabeth Bovenmyer

  2. Structure • DH Production in Maize: How ? • Why (Doubled) Haploids ? • Who uses DHs ? • Need for a Public DH Facility ?

  3. Technologies • Haploid production • - In vitroversusin vivo • - Maternal versus Paternal • 2) Haploid identification • Markers: Morphological or molecular • Cytological / flowcytometry • 3) Genome doubling • Colchicine • Other

  4. In vitro: Anther Culture

  5. In vivo Haploid Induction F1 X X Paternal Inducer (ig) Maternal Inducer + + X , , , , , , F2

  6. Workflow & Bottlenecks Maternal DH Induction

  7. Identification of Maternal Haploids Donor Inducer Endosperm Embryo Outcrossed or self-pollinated H embryo F1 embryo Lethal Geiger 2009

  8. Comparison DH Approaches in Maize

  9. Why (Doubled) Haploids ?

  10. Speed in Line Development Founder line 1 x Founder line 2 F1 DH Selfing Inbred line Inbred line

  11. Haploid Induction & Doubling of Allelic Diversity Materials Andrew Smelser et al., Plant Introduction Station, Ames

  12. QTL MAPPING

  13. Trait / Gene Stacking Line 1 Line 2 Probability for Fixation of Target Genes X or Selfing (F2) DH Induction Goal: Fixation of target alleles

  14. Usefulness in Line Development Seitz 2005

  15. Rapid Line & Population Improvement Gordillo & Geiger 2008

  16. Conclusions • Homozygous lines are obtained in short time • Maximal genetic variation among lines: increased selection gain • Reduces population size for gene pyramiding substantially • Increases power for QTL or QTP detection • Increases labor for line development – depending on technology

  17. Who uses DHs ?

  18. DH Application in Plant Breeding Maize: All major breeding companies (in vivo DH induction) Other species (Tuvesson et al.): Rye, Oat, Triticale, Potato, Cabbage Tuvesson et al. 2007, EU-COST

  19. Need for Public DH Facility ?

  20. Pro and Contra Public DH Facility (Maize) Contra: - Inducers are available in maize - Procedures are published -> Everyone can do it Pro: - Users might need DH materials only once or once in a while - Induction procedure requires know how (analogy to transformation) - Technology advance might make procedure more accessible - Possible platform for expansion into other species => Research opportunities in maize and other species And: Training of future plant breeders !! Technology & Application of DHs

  21. Technological Challenges Partial Fertile • Questions relating to inducer: • Alternative/improved marker systems • Increased induction rate • Adaptation to US climate / photoperiod • Genetics / biology of induction mechanism • Questions relating to genome doubling: • Genetic background effect ? • Optimized procedure (with colchicine) • Procedure without colchicine ? • Increased doubling / success rate ColoredSeed Smelser et al. 2009

  22. Conclusion Successful example of research oriented service facility at ISU: http://www.agron.iastate.edu/ptf/index.aspx New DH facility at ISU will soon be launched, if interested contact: Thomas Lübberstedt 515-294-5356 THOMASL@IASTATE.EDU Collaborators: Candy Gardner, Mike Blanco, Uschi Frei

  23. Costs ? • Summer / Winter pricing • GM seed ?

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