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Visiting IFE Wednesday 21th Mars?

Visiting IFE Wednesday 21th Mars?. Optical microscopy. MENA3100,OBK, 31.01.12. Optical microscope Optisk mikroskop. Light microscope Lysmikroskop. Magnification (forstørrelse) Resolution (oppløsningsevne) Contrast. We want to see more details. Simple microscope.

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Visiting IFE Wednesday 21th Mars?

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  1. Visiting IFE Wednesday 21th Mars?

  2. Optical microscopy MENA3100,OBK, 31.01.12

  3. Optical microscope Optisk mikroskop Light microscope Lysmikroskop Magnification (forstørrelse) Resolution (oppløsningsevne) Contrast We want to see more details

  4. Simple microscope Lupe, forstørrelsesglass

  5. The simple microscope Antony van Leeuwenhoek (1632−1723)

  6. Det sammensatte mikroskop The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635−1703) Micrographia, 1664

  7. Don’t get too hang up in the ray tracing

  8. Stereomikroskop Stereo microscope

  9. Mikroskop for gjennomfallende lys Compound microscope for transmitted light

  10. Mikroskop for reflektert lys Inverted compound microscope for reflected light

  11. www.microscopy-analysis.com/files/ jwiley_microscopy/2006_Sept_Hammond.pdf

  12. Illuminating system Specimen Condenser lens Aperture diaphragm Field diaphragm Filament

  13. Illuminating system Specimen Condenser lens Aperture diaphragm Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Field diaphragm Aperture diaphragm Controll angle of the cone of light Filament

  14. Imaging system Eyepiece Okular Tube lens Objective Specimen

  15. Primary image

  16. Refraction

  17. Kromatisk abberation

  18. Spherical abberation

  19. Corrections The objective

  20. The limiting factor: Diffraction

  21. Resolution R = 0,61l/NA Rayleigh criterion (Raleigh in Brandon and Kaplan)

  22. Abbe

  23. Numerical aperture (NA) NA = nsina Resolution R = 0,61l/NA

  24. Oil immersion

  25. Fouriertransformasjon

  26. More is needed than just the objective Condenser system Eyepice Ocular (okular)

  27. Optical anisotropy Calcite

  28. Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

  29. Optically active crystals in the microscope

  30. Optically active crystals in the microscope

  31. Michel-Levy chart

  32. Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/50-1-0011_e.pdf

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