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Ochratoxin A (OTA)

Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography. Toxicity OTA is hepatotoxic, nephrotoxic, teratogenic and carcinogenic to animals

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Ochratoxin A (OTA)

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  1. Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography • Toxicity • OTA is hepatotoxic, nephrotoxic, teratogenic and carcinogenic to animals • OTA was classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen (group 2B) Regulatory limits set by the European Union • COMMISSION REGULATION (EC) No 123/2005 • 2,0 ppb for wine (red, rose and white) Ochratoxin A (OTA)

  2. Sample preparation and clean-up Method • Chromatographic determination • The clean-up procedure for ochratoxin A involves generally IAC, SPE • Immunoaffinity chromatography (IAC) clean-up is based on the binding interaction of ochratoxin A-specific antibodies and has been set as European Standard (SR EN 14133) for ochratoxin A analysis in wine and beer Immunoaffinity column design containing solid extraction phase made from antibody-modified support beads binding ochratoxin A

  3. Sample preparation and immunoaffinity clean-up

  4. HPLC determination of ochratoxin A • Chromatographic conditions: • OTA was quantified by reverse phase high–performance liquid chromatography (HPLC) with fluorescence detection (excitation wavelength 330nm, emission wavelength 450nm). • mobile phase consisted of a mixture of acetonitrile–water–acetic acid (99:99:2) • analytical column were reverse phase, C-18 with 5 μm particles • flow-rate 1 mL/min • Injection 20 yL Chromatogram of OTA contaminated red wine

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