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Zooming in on human serum: increasing proteomic coverage using a 2-D gel approach

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Zooming in on human serum: increasing proteomic coverage using a 2-D gel approach

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    1. Zooming in on human serum: increasing proteomic coverage using a 2-D gel approach E. McGregor Proteome Sciences plc, London, UK.

    2. Proteomic analysis of serum/plasma for biomarker discovery

    3. Proteomic techniques

    4. Major plasma proteins

    5. Dynamic range of the human plasma proteome

    6. Increasing proteomic coverage: affinity depletion of albumin, Ig and other major serum/plasma proteins

    7. Increasing proteomic coverage: affinity depletion of albumin and IgG

    8. Increasing proteomic coverage: affinity depletion of albumin, IgG and other major plasma proteins

    9. Pre-fractionation by solution phase IEF

    10. Pre-fractionation by solution phase IEF: Invitrogen ZOOM IEF Fractionator

    11. Fractions of undepleted serum on 2DE

    12. Fractions of depleted serum on 2-DE Zoom IPG gels

    13. Protein identification via MALDI-TOF & LC-MS/MS: depleted fractions

    14. Protein identification via MALDI-TOF & LC-MS/MS: bound fractions

    15. Conclusions

    16. Acknowledgements

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