180 likes | 376 Views
Hanyang Univ. BET surface area vs polymerization time. Hanyang Univ. Pore Size(nm) vs polymerization time. Hanyang Univ. Pore Volume vs polymerization time. Hydrothermal Treatment. Liquid Phase(various solvent) - Immersioning silica particle Gas Phase -Nitrogen -Steam
E N D
Hanyang Univ. BET surface area vs polymerization time
Hanyang Univ. Pore Size(nm) vs polymerization time
Hanyang Univ. Pore Volume vs polymerization time
Hydrothermal Treatment • Liquid Phase(various solvent) - Immersioning silica particle • Gas Phase -Nitrogen -Steam • dehydration or dissolution • -Silica dissolves from fine particle
Illustration of pore structure before (a) and after (b) hydrothermal treatment by means of autoclave process Hanyang Univ. Inorganic Advanced Material Lab
- Hydrothermal process Reactor(High Pressure) -Temp. , Time control Centrifuge (Treated gel + Water) Washing (DI water) Vacuum drying Grinding Classification Characterization Hanyang Univ. Inorganic Advanced Material Lab
Immobilization of Enzyme - Schematic procedure for the silanization of silica Silica(10g) Washing(with methanol, H2O2, HNO3) Adding aminosilanes(50ml) Agitating(110C *4hrs) Washing with DW Drying(105C *2hrs) Hanyang Univ. Inorganic Advanced Material Lab
- Schematic procedure for the immobilization of DAAO Silanized silica(10g) Adding of 25% Glutaraldehyde(30ml)/ 200ml of 0.1M potassium phosphate buffer (pH7.5) Agitating(20C *1hr) DW washing Suspending in 300ml of 0.1M potassium phosphate buffer (pH7.5) Adding of DAAO solution (200U/ml*5ml) Agitating(20C *1hr) Immobilized yield by HPLC Hanyang Univ. Inorganic Advanced Material Lab
(b) (a) Fig. 7. Effect of pore size on the amount (a) and activity (b) of immobilized enzyme ■ : pH 4.0, Temp. 70℃ ● : pH 9.0, Temp. 70℃
Study on Biocatalyst support Immobilization yield vs. pore diameter. Covalent Immobilization of D-amino acid oxidase to Nano-porous silica. 3APTES (3-aminopropyltriethoxysilane)