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Cross-border network for knowledge transfer and innovative development in wastewater treatment WATERFRIEND HUSRB/1203/221/196 1st HUSRB Students Meeting. . . 1st Students Meeting Waterfriend . . .

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  1. Cross-border network for knowledge transfer and innovative development in wastewater treatmentWATERFRIENDHUSRB/1203/221/1961stHUSRB Students Meeting

  2. . 1st Students Meeting Waterfriend . Bioethanol production from cellulosic and hemicellulosic agricultural waste Dóra Vitay Bioengineering Bsc. student Supervisor: prof. Dr. Cecília Hodúr Co-supervisor: Marietta Ábel

  3. . 1st Students Meeting Waterfriend . Objectives • Howtobaccocan be usedas a feedstockforbioethanolproduction • Cellulose and hemicelluloseenzymaticdegradation: • Withcellulase and cellobiasemixtureondifferent pH • Withxylanase • Possibility of enzymerecycling • Membraneseparation • Monitoring theactivity of used and separatedenzymes Tobacco plant

  4. . 1st Students Meeting Waterfriend . Materials and methods • Tobacco: (Nicotianarustica, Nicotianatabacum) • Usuallylivesintropicorsubtropicalzone, angiospermplant • 1-2 metershigh, goodadaptibility • Usedsamples: • Experimental  leaves + vines • By-product  remainingvinesaftermanufacturingprocess • Grinding • Fermentationinshakingwaterbathwithcellulase (Asp. niger), cellobiase (Trichodermareesei) and xylanaseenzymes • Reducingsugaranalysiswith 3,5-dinitrosalicylic acid (DNSA) Samplebeforegrinding Sampleaftergrinding

  5. . 1st Students Meeting Waterfriend . Materials and methods • Ultrafiltration: (Millipore) • 5 KD, PES (poliethersulfone) membrane • 3.5 bar • Ultrasonicwavegenerator • Reusedenzymeactivityinspection • „filter paper” / avicell test Shakingwaterbath Ultrafilrtationequipmentwithultrasonicwavegenerator

  6. . 1st Students Meeting Waterfriend . pH comparison • Circumstances: • pH = 5.0 and 4.8 • T = 50°C • Sampling and pH settinginevery 24 hours • Reducingsugaranalysisoneverysample Experimental tobaccosamplesafterfermentation

  7. . 1st Students Meeting Waterfriend . pH comparison

  8. . 1st Students Meeting Waterfriend . Results of degradationwithxylanase Experimental tobaccosamplesafterfermentation • Circumstances: • pH = 4.5 • T = 30°C • Samplingand pH settinginevery 24 hours • Reducingsugaranalisysoneverysample( Experimental tobaccosamplesduringreducingsugaranalysis

  9. . 1st Students Meeting Waterfriend . Results of degradationwithxylanase

  10. . 1st Students Meeting Waterfriend . Results of membraneseparation • Circumstances: • 3.5 bar • 350 rpm • Membrane: 5KD PES • Ultrasoundoccasionally Fluxequation Resistanceequation Byproductsamples: feed, concentratum, permeatum

  11. . 1st Students Meeting Waterfriend . Results of membraneseparation

  12. . 1st Students Meeting Waterfriend . Results of membraneseparation

  13. . 1st Students Meeting Waterfriend . Results of enzymerecycling • Circumstances: • pH = 5.0 • T = 50°C • Sampling and pH settinginevery 24 hours • Reducingsugaranalisysoneverysample

  14. . 1st Students Meeting Waterfriend . Conclusion • The main goals of ourexperimentsweretostudythepossibilitiesoftheby-producttobaccousage • As a feedstockforbioethanolproduction • First, weusedcellulase and cellobiasemixtureandxylanaseenzymestohidrolyzethesamples • Both enzymaticwaysareabletodegradethetobacco • Therewas no significantdifferencebetween pH 4.8 and 5.0 • Xylanasewas more effectivethancellulase-cellobiasemixture • Furtherstudiesshowed, thatmembraneseparation is a possiblemethodtoregaintheenzymes • Afterseparationtheenzymeskeepedtheiractivity, evenaftertheultrasoundtreatment, butwithlesseractivity

  15. . 1st Students Meeting Waterfriend . Thankyouforyourkindattention!

  16. . 1st Students Meeting Waterfriend . Acknowledgements I wouldliketothanktomysupervisor prof. dr. Cecília Hodúr and myco-supervisor Marietta Ábel. • Jelen eredmények megjelenését „Zöld Energia Felsőoktatási Együttműködés (ZENFE)” című, TÁMOP-4.1.1.C-12/1/KONV-2012-0012 azonosítószámú projekt támogatja. A projekt az Európai Unió támogatásával, az Európai Szociális Alap társfinanszírozásával valósul meg. • A szerzők köszönetet mondanak az Országos Tudományos Kutatási Alapprogram pénzügyi támogatásáért. A projekt azonosító száma: K 105021. Presentation/lecture has been produced with the financial assistance of the European Union. The content of the presentation/lectureis the sole responsibility of University of Novi Sad, Faculty of Technology and can under no circumstances be regarded as reflecting the position of the European Union and/or the Managing Authority.

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