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Program

Program. Interactions. Epigenetics. Environmental «triggers». Genetics. Nutrition Lifestyle Stress Infections Microbiota ++++. You. N europeptides in urine LC- MS / MS. Dag Tveiten Adrian Finvold 2013. Peptides to nervous system. Incomplete breakdown of gluten and casein

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Program

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  1. Program

  2. Interactions Epigenetics Environmental «triggers» Genetics • Nutrition • Lifestyle • Stress • Infections • Microbiota • ++++ You

  3. Neuropeptides in urineLC- MS/MS Dag Tveiten Adrian Finvold 2013

  4. Peptides to nervous system • Incomplete breakdown of gluten and casein • Permeability – “Leaky gut syndrome” • Pathway • Diet and effect

  5. UV- spectrum of urine • UV-spectrum of urine with given wavelengths

  6. Derek - 27 • Difficult birth – normal weight • Reduced weight soon after birth • 9,5 kg when 2,5 years old • Diagnosed with Infantile Autism - 4 years old • Often GI symptoms – pain, diarea • Angry often/aggressive behaviour

  7. Derek ? Derek 2007 – 64 kg Derek 2005 – 50 kg With permission from parents

  8. Derek Gluten- and casein free diet Before GFCF diet: • Rigid, stiffness, unrelaxed • Hardly no eye contact • Aggressive • Tramping with his feet • Biting and kicking After GFCF diet: • More relaxed • Smiling more often • More happy • Eyecontact • More communicative Parents say: One hour after ingestion of ”forbidden” food – marked increased aggressive behaviour

  9. Publications

  10. Project LC-MSMS • Autumn 2011 • Investment Ms • Cooperation with HIOA, RH (Medinova) • 4 students Bachelor in Chemistry

  11. Peptide structures • Casomorphine 1-4 Gluten exorphine C - Mass: 522,2 g/mol - Mass: 591.3 g/mol

  12. Enzymatic degrading effects

  13. Calibration curveRelation between signal and concentration

  14. Mass spectrometry Conclusions • Fast • Selective • Sensitive • Reliable

  15. Mass spectrometry Patient - LC-MsMs

  16. Mass spectrometry Patient

  17. Patient example • Patient lab results – Antibodies/Peptides IgGcowmilk 68 U/mL (<20) IgGcasein 40 U/mL (<20) IgAcasein 13 U/mL (<12) Casomorphin 1-4 41,4 nmol/L/kreatinin Casomorphin 1-5 45,2 nmol/L/kreatinin Casomorphin 1-7 200,1 nmol/L/kreatinin Casomorphin 1-8 145,2 nmol/L/kreatinin

  18. E-mails from Kalle Vi får ta en titt På det som er blitt Et enigma i en gåte Som nålen i en såte. Hi ,hi Kalle Hurra.hurra Det er bra .bra .bra. Dette går veien Som tosken og skreien . VH  K Hipp hurra Dette var bra. Nå har vi seiret , Og dette må feires VH  kalle den nå helgale

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