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1999

2004. 2000. 2011. 2012. 2013. 2014. 2001. 2003. 2005. 2006. 2008. 2010. 2007. 1999. 2009. 2002. Other schools. 217. BIOlab Visits. 362. 1731. 1152. 2647. 2201. In-School Visits. 446. Other schools. 2012-2013. Why youth?. Programming and Curriculum.

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1999

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  1. 2004 2000 2011 2012 2013 2014 2001 2003 2005 2006 2008 2010 2007 1999 2009 2002

  2. Other schools 217 BIOlab Visits 362 1731 1152 2647 2201 In-School Visits 446 Other schools 2012-2013

  3. Why youth?

  4. Programming and Curriculum • Grade 5: Maintaining a healthy body • Grade 6: Health curriculum • Grade 7: Solutions, ecosystems • Grade 8: Cells and systems, optics, fluids • Grade 9: Reproduction • Grade 11 Biology: Body systems • Grade 12 Biology: Genetics • Developed with teachers • Based on MB curriculum • Hands-on, authentic science

  5. Lab Safety Basics • If you don’t know what it is, don’t touch it! • No food or drinks! • Wear safety equipment when instructed! • Follow instructions! • Ask questions if you don’t understand instructions!!!

  6. Lab Safety Basics • You must wash your hands when leaving the lab and after removing your gloves! • Report any spills immediately!

  7. Lab Safety Basics • You will be wearing gloves for a reason! DO NOT touch other people or any surfaces when wearing gloves!

  8. Your patient Patient was brought in by his wife after he fainted at home. He is complaining of severe chest pain.

  9. Tests You take his blood pressure: BP= 168/93 You listen to his heart: Sound 1 Sound 2

  10. EKG You order an EKG: this can show us if his heart is beating properly. normal patient

  11. Echocardiography Turbulent flow around aortic valve Left ventricular hypertrophy

  12. Diagnosis: Aortic Stenosis Normal aortic valve Stenotic aortic valve

  13. What now? CT Scan Valve replacement surgery

  14. Or could we build him a new valve? Get a donor valve Decellularize donor valve Get stem cells from patient Grow stem cells from patient into the cell type we want

  15. Stem cells Embryonic Adult From bone marrow or possible other sites Any type of cell! Some types of cells!

  16. Stem cell therapy • Grow billions of cells • Push to cell type we want

  17. Muscle cells (myocyte) Fibroblasts and myofibroblasts Cell culture Digest Research

  18. Working with live cells • Using a dropper, suck out the media (liquid cell food), discard in waste beaker • Using a clean dropper, cover with PBS and gently wash cells by rocking the dish • Remove PBS and discard in waste tube. Using a clean dropper each time, do two more PBS washes and discard. Check cells under scope when done. • Using a clean dropper, add 2ml trypsin and rock in an ‘X’ pattern until cells are detached (check on the scope, about 5min) • Using a clean dropper, add 2ml DMEM and draw up all of the liquid with the dropper DO NOT PUT INTO THE WASTE CONTAINER!!! • Put the liquid into a clean, empty 15ml blue capped tube • Wait for instructions to spin in centrifuge

  19. Next steps • We hope to partner with your school division! Division consultants are attending the BIOlab in November, please get in touch with yours to express interest. • Get in touch with Steve sjones@sbrc.ca • Check out the website www.youthbiolab.ca

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