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Suspended animation-like state protects from lethal hypoxia Journal Club of Life Sciences

Suspended animation-like state protects from lethal hypoxia Journal Club of Life Sciences 27 th May 2010 Brian Lassen. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372. Suspended animation-like state protects from lethal hypoxia.

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Suspended animation-like state protects from lethal hypoxia Journal Club of Life Sciences

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  1. Suspended animation-like state protects from lethal hypoxia Journal Club of Life Sciences 27th May 2010 Brian Lassen Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia

  2. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia

  3. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia

  4. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Reducing power produced here due to reveresed electron flow driven by H+ gradient S2-, SO3-, NH3 provide electrons (S2O3-, Fe) H+ gradient formed here due to H+ pumped out of the cell during electron transfers O2- and S2- similar properties

  5. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Reducing power produced here due to reveresed electron flow driven by H+ gradient S2-, SO3-, NH3 provide electrons (S2O3-, Fe) H+ gradient formed here due to H+ pumped out of the cell during electron transfers Sulphur used to be terminal electron acceptor for respiration

  6. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Hypothesis Is sulphide a critical buffer in oxygen consumption after exposure to hydrogen sulphide?

  7. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Can pre-exposure to H2S increase survival in lowered amounts of oxygen? Method Result Pretreatment 150 ppm H2S and 20.3% O2, 20 min 5% O2 No Pretreatment 20.3% O2 5% O2 Figure 1 Normal survival is 15 min. (LD50)

  8. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Can continued exposure to H2S increase survival and metabolic rate in lowered amounts of oxygen? Method Result 80 ppm H2S and 5% O2, 6 hours Pretreatment 150 ppm H2S and 20.3% O2, 20 min Figure 2 5% O2, 6 hours 50 fold drop in metabolic rate

  9. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Can mice survive in less than 5% oxygen after pre-exposure? Method Result PT 150 ppm H2S and 20.3% O2, 20 min PT +1 150 ppm H2S and 20.3% O2, 20 min No PT 20.3% O2, 20 min 5% O2, 1 hour Figure 3 5% No PT 5% O2, 1 hour 5% PT 5% O2, 1 hour 4% PT 4% O2, 1 hour 4% PT+1 4% O2, 1 hour 3% PT+1 3% O2, 1 hour No animals survived All animals survived

  10. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Metabolic markers: CO2 production during hypoxia correlate with survival time? Comparing 5 min. before 5% oxygen exposure to 5 min. after. Method Result PT 150 ppm H2S and 20.3% O2, 20 min PT +1 150 ppm H2S and 20.3% O2, 20 min No PT 20.3% O2, 20 min Figure 4 5% O2, 1 hour 5% No PT 5% O2, 1 hour 5% PT 5% O2, 1 hour 4% PT 4% O2, 1 hour 4% PT+1 4% O2, 1 hour Figure 4 All animals survived No animals survived CO2 reduction seems important to survival

  11. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia Conclusions & Discussion Pre-exposure to H2S seems to increase survival rate of mice in lethan (5%) hypoxia Pre-treatment in 5% oxygen levels lowers the metabolic rate and enables survival at even lower levels of oxygen The process of putting mammals in an animated state at low levels of H2S is reversable without apparent side effects Perhaps COX can be considered an area of supply and demand in aerobic cells rather than create an imbalance (producing reactive oxygen species)

  12. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia What is next? Thought developed further to put mammals in animated state after lethal injuries increasing survival chances by expanding the response period The research team created a liquid based dose of H2S to put mammals into animated state in case of emergencies Experiments are currently (2010) in second phase human trails

  13. Blackstone, E; Roth, M.B., 2007. Shock, vol. 27, No. 4, p 370-372 Suspended animation-like state protects from lethal hypoxia www.ted.com Mark Roth

  14. Next Journal Club of Life Sciences Tõiv Haljasorg Mass spectrometry, Part II 10th June 2010

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