1 / 21

Polymorphism of Ralstonia solanacearum and it’s plant vaccin development

Polymorphism of Ralstonia solanacearum and it’s plant vaccin development. Prof. Dr. Liu Bo Agricultural Microbiology Research Team Bioresources research institute Fujian Academy of Agricultural Sciences fzliubo@163.com. 1. 2. 3. 4. 5. Bacterial wilt disease and its pathogen forms.

trory
Download Presentation

Polymorphism of Ralstonia solanacearum and it’s plant vaccin development

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Polymorphism of Ralstonia solanacearum and it’s plant vaccin development Prof. Dr. Liu Bo Agricultural Microbiology Research Team Bioresources research institute Fujian Academy of Agricultural Sciences fzliubo@163.com Agricultural microbiology research team

  2. 1 2 3 4 5 Bacterial wilt disease and its pathogen forms Polymorphism of Ralstonia solanacearum (RS) Characteristics of avirent RS Construction and mechanism of avrient RS New idea of plant vaccin development Contents FJAT

  3. 1、Bacterial wilt disease and its pathogen forms (1) About bacterial wilt disease Ralstonia solanacearum was found in 1864 ，it could infected many plants with about 44 family，more than 300 species，so called “plant cancer”. Many researches has been made in the population dynamic, subspecies variation, molecular biology, gene regulation, etc.。 But the disease control would remain difficulties . The new idea was put forward to develop plant vaccin. FJAT

  4. 1、Bacterial wilt disease and its pathogen forms (2) RS existed the virulent and avirulent strains, identified by TTC culture medium and plant infection. RS Differentiation to Virulent St. Avirulent St. Infection and dead Infection and alive FJAT

  5. 1、Bacterial wilt disease and its pathogen forms (3) Pathogenicity index [PI] of virulent and avirulent RS in TTC medium virulent interim avirulent Mortality (%) Pathogenicity index Pathogenicity index was made up by red diameter / whole colony diameter RS strains were collected from fields with the relationship between pathogenicity index and mortality. FJAT

  6. G1: High infected mortaliy, short latent period, small PI <0.41 G2: Middle to high infected mortaliy, long latent period, middle PI=0.52-0.68 。 。 。。 。 。 。 。 。 。 。 。 G3: No infected mortaliy, long latent period, large PI>0.80 1、Bacterial wilt disease and its pathogen forms (4) Pathogenicity divergency of RS populations infected in tissu culture plantlet of tomato FJAT

  7. 2、Polymorphism of Ralstonia solanacearum (RS) (1) RAMS primers polymorphism analysis of Ralstonia solanacearum RAMS primers polymorphism tomato Pu tian place egg plant Cluster analysis based on the RAMS bands showed that 22 isolates could be divided into 3 group with characters of host crop and climate zones. Ning De place pepper FJAT

  8. λ=20 2、Polymorphism of Ralstonia solanacearum (RS) (2) BOX-PCR geographic polymorphism analysis of R. solanacearum Tomato plants Group 1 Ningde dist. The cluster analysis showed that the BOX-PCR genetic diversity of R. solanacearum was concerned on geogra- phic origins with group 1 for Ningde district, group 2 for Nanping dis-trict, group 3 for Fuzhou district. Group 2 Nanping dist. Group 3 Fuzhou dist. FJAT

  9. λ=8 2、Polymorphism of Ralstonia solanacearum (RS) (3) REP-PCR hostpolymorphism analysis of R. solanacearum Ningde district Group 1 Egg plan+tomato The cluster analysis showed that the REP-PCR genetic diversity of R. solanacearum was concerned on host with group 1 for Egg plan+tomato , group 2 for Pepper, group 3 for Pepper+tomato. Group 2 Chilli Group 3 Chilli+tomato FJAT

  10. 2、Polymorphism of Ralstonia solanacearum (RS) (4) PLFAspolymorphism analysis of R. solanacearum PLFAs of the virulent and avirulent RS The PLFAs of A, B, C, D, E, F, bet-ween the aviru-lent and virulent RS to show the avirulent with 0% M., virulent with 30% M. and high toxicity with 100% M. Avirulent Virulent FJAT

  11. 2、Polymorphism of Ralstonia solanacearum (RS) (5) Discriminationanalysis of PLFAs to distinguish the pathogenicity RS Y1=-16.3353 + 0.0012X1 + 0.0056X9 - 0.0016X13 Y2= -3.4928 + 0.0002X1 + 0.0003X9 + 0.0025X13 Y3= -9.1550 - 0.0003X1 + 0.0039X9 + 0.0042X13 G1：avirulent X1=C14:0, X9=C17:0, X13=C18:0 G2：interim group 1 - avirulent strains with PI>0.80，reinfected mortality 0% in the tomato tissue culture plantlets . group 2 - interim strains with PI in the range of 0.60-0.80, reinfected mortality 6.7%~ 100% in the tomato tissue culture plantlets。 group 3 – virulent strains with PI<0.60, reinfected mortality 100% in the tomato tissue culture plantlets. G3：virulent Cluster of the RS strains based on PLFAs FJAT

  12. 2、Polymorphism of Ralstonia solanacearum (RS) (6) Genome analysis of the virulent and avirulent RS strains FJAT

  13. Diagram Company History 2003 2000 2003.10 Add Your Text 2003.10 Add Your Text 2003.10 Add Your Text 2002 2000.10 Add Your Text 2000.10 Add Your Text 2000.10 Add Your Text 2002.10 Add Your Text 2002.10 Add Your Text 2002.10 Add Your Text 2001 2001.10 Add Your Text 2001.10 Add Your Text 2001.10 Add Your Text FJAT

  14. 3、Characteristics of avirent RS (1) Some characteristics of virulent and avirulent RS Avirulent strain Virulent strain Concentration (CFU/ml) Cultural time virulent avirulent EPS ug/ml Control medium virulent avirulent FJAT

  15. 3、Characteristics of avirent RS (2) Factors effecting on the change from virulent to avirulent RS CHEM: Antibiotic can’t change RS to avirulent PHYS: Ultrasonic can’t change RS to avirulent BIO: Bacillus can change RS to avirulent Subculture can’t change RS to avirulent CUL: Cultural time can’t change RS to avirulent FJAT

  16. 3、Characteristics of avirent RS (3) Inhibit machenism of Bacillus cereus to v-RS and a-RS Fermentation liquid (X4) of Bacillus cereus caused 99% mortality to virulent strain, 37% mortality to avirulent strain. It meant that the virulent strain was more susceptible to Bacillus cereus than the avirulent did. FJAT

  17. 3、Characteristics of avirent RS (4) Simulation of inhibit effect of Bacillus cereus to v-RS and a-RS 1 3 4 2 Pure v-RS(1) and a-RS(2) mixed with B. cereus respectively resulted in (3) with two strains growing together and in (4) with only a-RS gowing. It meant that B. cereus could select the RS avirulent strain and suppress the RS virulent strain. FJAT

  18. 4、Construction and mechanism of avrient RS FJAT

  19. Text2 Text3 Text1 Text4 Text5 4、Construction and mechanism of avrient RS FJAT

  20. Concept concept Concept Concept 4、Construction and mechanism of avrient RS Add Your Text Add Your Text FJAT

  21. www.themegallery.com Click to edit company slogan . Thank You ! Agricultural microbiology research team

More Related