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Monounsaturated Fatty Acid Modification of Wnt Protein: Its Role in Wnt Secretion

Monounsaturated Fatty Acid Modification of Wnt Protein: Its Role in Wnt Secretion. Takada R, Developmental Cell (2006). Ciani L, Nature (2005). Coudreuse D, Development (2007). Wntless/Evi, a seven-pass membrane protein, plays an essential role in Wnt secretion.

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Monounsaturated Fatty Acid Modification of Wnt Protein: Its Role in Wnt Secretion

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  1. Monounsaturated Fatty Acid Modificationof Wnt Protein: Its Role in Wnt Secretion Takada R, Developmental Cell (2006)

  2. Ciani L, Nature (2005)

  3. Coudreuse D, Development (2007)

  4. Wntless/Evi, a seven-pass membrane protein, plays an essential role in Wnt secretion. Banziger C, Cell (2006) and Bartscherer K, Cell (2006)

  5. The retromer complex, participates in the long-range transport of Wnt protein Coudreuse D, Development (2007)

  6. - Wnt is a family of cysteine-rich secreted glycoproteins. Mason JO, Mol Biol Cell (1992) - Wnt proteins are lipid-modified (palmitoylated). Willert K, Nature (2003)

  7. Porc - The Porcupine (Porc) may be involved in secretion or ER transport and has some homology to a family of O-acyl transferases and may be involved in lipid modification of Wnt proteins. Linder ME, Journal of Cell Science (2004)

  8. - Wg secretion is inhibited in the absence of Porc, whereas Wnt3a (C77A) is secreted normally. - According to mass spectrometry analysis of Wnt-3a, there remains the possibility of additional acylation sites.

  9. Ser209 Is Required for Acylation of Wnt-3a.

  10. Wnt-3a Is Modified with a Monounsaturated Fatty Acid at Ser209

  11. Ser209 Is Essential for Secretion of Wnt-3a

  12. Porcupine Is Required for the Acylation and Secretion of Wnt-3a

  13. Wnt-3a with Defective Acylation Is Not Transferred from the ER

  14. Ser209 Is Required for the Function and Appropriate Localization of Wnt-3a in Xenopus Embryos

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