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Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration

Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration . Holim Jang , Hong Ryul Ahn , Hyoung Jo , Kyung-A Kim , Eun Ha Lee , Ki Won Lee , Sang Hoon Jung , and Chang Y. Lee . The Retina. Photographic film of the eye Retinal ganglion (RGC-5) cells

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Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration

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  1. Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration Holim Jang, Hong RyulAhn,HyoungJo, Kyung-A Kim, Eun Ha Lee, Ki Won Lee, Sang Hoon Jung, and Chang Y. Lee

  2. The Retina • Photographic film of the eye • Retinal ganglion (RGC-5) cells • Axons form optic nerve

  3. Hypoxia • Metabolically active • Oxidative stress • Reduction in oxygen supply • Risk factor for sight-threatening disorders

  4. Nitric Oxide (NO) • Hypoxia  NO binds toComplex IV O2- formed • Formation of peroxynitrite (oxidizing agent) • NO + O2- ONO2-

  5. Chlorogenic Acid (CGA) • Antioxidant phytochemical • Major source is coffee • High blood absorption rate (33%) • Green coffee

  6. Question • Do CGA and coffee protect against retinal degeneration? • In vitro & in vivo

  7. Method: In Vivo • Male mice 30-35 kg • Optic nerve crush • Left: crush • Right: control • CGA & coffee extract (oral)

  8. Method: In Vivo • RGC labeling: superior colliculi exposed • Fluorogold • Eyeballs enucleated after 5 days • Retinal flat mounts

  9. Method: Analysis • Hematoxilin & Eosin staining • Nuclei = blue • Cytoplasm & proteins = pink • TUNEL staining • Apoptosis = brown

  10. Results: CGA

  11. Results: CGA

  12. Results: CGA

  13. Results: CGA

  14. Results: Coffee Extract

  15. Results: Coffee Extract

  16. Results: Drawing Conclusions • CGA’s NO scavenging activity • Optic nerve damage  inflammatory response  NO release • Supported by in vitro

  17. Discussion • Retinal degeneration from hypoxia • CGA = NO scavenger • Limitations • Mice • CGA concentration

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