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Figure 1

A. B. C. D. A. B. 10. 4. 6. 9. 7. 5. 2. 10. 9. 5. 6. 3. 11. 1. 8. 7. 8. 11. 2. 5. 3. 1. 4. Figure 1.

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Figure 1

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  1. A B C D

  2. A B 10 4 6 9 7 5 2 10 9 5 6 3 11 1 8 7 8 11 2 5 3 1 4

  3. Figure 1 • 1a. Flowcell schematic. PDMS mold forms channel for analyte flow-through. Mounted on temperature controlled plate, cell has cutouts for tapered fiber and microsphere. Sample is injected via vertical port and vacuum port removes waste. Flow-cell integrated biosensor. Tapered fiber runs through PDMS and microsphere enters through cutout in mold. Sample is injected through side port (yellow) and buffer is flowed through vertical (black). Waste is collected through vacuum drainage system (green). Total volume is ~100 μl. The entire sensor sits on Peltier controlled plate, regulating temperature to 0.01° C. • 1b. Current system setup. • 1c. Syringe pump controller and setup. • 1d. System diagram (for David)

  4. Figure 2 • 2a. MS2 virus detection experiment. Includes surface treatment to covalently immobilize antibody onto glass sphere. Steps 1-4 include surface modification. Phix174 was introduced first followed by a wash with PBS, followed by glycine surface generation. MS2 phage.was introduced after baseline was stabilized again. • 2b. Zoomed in version of 2a highlighting Phix174 removal and MS2 virus binding. Phix174 was injected ( • 2c. Same surface modification technique; MS2 introduced first (1), followed by PBS wash (2) and regeneration with glycine (3). Phix174 (4) was added next followed by PBS wash (5).

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