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Sonia Gauthier and Michel J. Tremblay

Sonia Gauthier and Michel J. Tremblay. Introduction: GALT and HIV-1. Transmission Mother-to-child Intestinal CD4 depletion during primo-infection Majority of CD4-T cells are CCR5 positive 70% Viral reservoir Viral RNA detectable on successful HAART. Cytokines in the GALT environment.

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Sonia Gauthier and Michel J. Tremblay

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  1. Sonia Gauthier and Michel J. Tremblay

  2. Introduction: GALT and HIV-1 • Transmission • Mother-to-child • Intestinal • CD4 depletion during primo-infection • Majority of CD4-T cells are CCR5 positive • 70% • Viral reservoir • Viral RNA detectable on successful HAART

  3. Cytokines in the GALT environment

  4. Effect of different cytokines on HIV-1 replication in HT-29 cells ** ***

  5. Effect of different cytokines on HIV-1 transactivation in HT-29 cells *** *** ***

  6. IL-4 modulates GalCer and CXCR4 expression on HT-29 cells ** *** GalCer expression CXCR4 expression

  7. IL-4 differently modulates VSV-G and NDK-Env-mediated virus entry * ** NDK-Env pseudotyped virus VSV- G pseudotyped virus

  8. IL-4 does not affect VSV-G pseudotyped viruses fusion ***

  9. IL-4 inhibits VSV-G pseudotyped viruses * *** *** ***

  10. IL-4 inhibits retrotranscription of VSV-G pseudotyped viruses *** ***

  11. Conclusions • IL-1α, IL-β and TNF-α stimulated viral transcription in HT-29 cells • IL-4 inhibits HIV-1 replication in HT-29 cells • Downregulation of GalCer and CXCR4 • Inhibition of retrotranscription or earlier step in viral cycle • Inhibition is dose-dependent • Maximal inhibition at 1U/ml (35pg/ml)

  12. Acknowledgements • Dr Michel J. Tremblay, Principal investigator • All Dr Tremblay team members • Dr Corinne Barat • Dr Michel Ouellet • Dr Mélanie Tardif • CIHR Doctoral Research Award

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