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Department of Microbiology & Immunology MIMM 323 Microbial Physiology and Genetics

Department of Microbiology & Immunology MIMM 323 Microbial Physiology and Genetics. J.W. Coulton, Ph.D., Professor james.coulton@mcgill.ca. Assigned reading. Lengeler et al. (1999) Chapter 1, p. 1-17 Bacteriology………. Clostridium difficile. Canadian Medical Association Journal www.cmaj.ca

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Department of Microbiology & Immunology MIMM 323 Microbial Physiology and Genetics

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  1. Department of Microbiology & ImmunologyMIMM 323Microbial Physiology and Genetics J.W. Coulton, Ph.D., Professor james.coulton@mcgill.ca

  2. Assigned reading • Lengeler et al. (1999) • Chapter 1, p. 1-17 • Bacteriology………..

  3. Clostridium difficile • Canadian Medical Association Journal • www.cmaj.ca • July 6; August 31, 2004 http://www.cmaj.ca/cgi/reprint/171/1/27 http://www.cmaj.ca/cgi/reprint/171/5/466

  4. Microbial Physiology • Conflicting character of micro-organisms • Cause disease • Destroy useful products • Foods • Computer chips • Jet fuel • Alter ecosystems • Kill/control detrimental micro-organisms

  5. Microbial Physiology • Conflicting character of micro-organisms • Food production • Solve problems • Environment: bioremediation • Agriculture: GMF • Medicine: recombinant vaccines • Exploit potential of beneficial m-o

  6. 3 Questions • How do micro-organisms find / transport nutrients? • How do micro-organisms convert nutrients into cellular components? • How do micro-ogranisms respond to changes in type / availability of nutrients?

  7. Bacterial cell architecture • Major structural and functional components of micro-organisms • Light microscopy of bacteria • Rod shape • Coccus shape • Spiral shape

  8. Staining • Gram stain • Differentiate groups of bacteria • Gram positive • Gram negative

  9. Nomenclature Rods: Escherichia coli, Bacteriodes fragilis, Bacillus subtilis Coccus: Staphylococcus aureus, Streptococcus pneumoniae, Sarcina lutea Spiral: Aquaspirillum serpens, Treponema pallidum, Borrelia bergdorferi

  10. Electron microscopy • Different surface organizations: • Thick, undifferentiated • Multilaminated • Exotic decorations: RS or S layers external to Gram-negative or Gram-positive cells • capsule

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