1 / 11

S tabilizing Im7 mutants map to its interface with E7

S tabilizing Im7 mutants map to its interface with E7. PenV R. PenV S. Stabilization of protein or Better folding environment. good folding active -lactamase. poor folding degradation. Spy Cradle. Normal pathway. SH. S. oxidation. S. Substrate proteins. SH. S. S.

tuyet
Download Presentation

S tabilizing Im7 mutants map to its interface with E7

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Stabilizing Im7 mutants map to its interface with E7

  2. PenVR PenVS Stabilization of protein or Better folding environment good folding active -lactamase poor folding degradation

  3. Spy Cradle

  4. Normal pathway SH S oxidation S Substrate proteins SH S S SH SH S S DsbA O2 DsbB Ubiquinone Cytochrome oxidases New pathway SH oxidation S S SH SH S S SH Thioredoxin Thioredoxin S S S Fe Fe S S S O2 membrane cytoplasm

  5. DsbC-like Mutant DsbG-K113E Reverses Charge to resemble DsbC DsbC DsbG wt DsbG-K113E

  6. MDH interactions with HdeA-F35W W35 + MDH lmax=336 nm - MDH lmax=346 nm

More Related