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Xiangpeng Li Russell School of Chemical Engineering The University of Tulsa

Influence of temperature and light quality on algae growth kinetics and nutrient composition. Xiangpeng Li Russell School of Chemical Engineering The University of Tulsa. This presentation focuses on three questions:. Why?. What?. How?. Algae bloom has long been an environmental problem.

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Xiangpeng Li Russell School of Chemical Engineering The University of Tulsa

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  1. Influence of temperature and light quality on algae growth kinetics and nutrient composition Xiangpeng Li Russell School of Chemical EngineeringThe University of Tulsa

  2. This presentation focuses on three questions: Why? What? How?

  3. Algae bloom has long been an environmental problem.

  4. Algae has also been used to clean water.

  5. Method to control harmful algae blooms(HAB). • Algaecide • Aeration • Mixing • Ultrasound

  6. Improve the water treatment efficiency.

  7. Algae has a long history of being harvested as food.

  8. Culturing conditions: temperature, medium, LED light. • Inoculation shaker • Batch culture with 250ml baffled flask

  9. Heterotrophic growth is 3 times higher than autotrophic growth Growth rate increases with temperature (24-32ºC)

  10. High biomass concentration under red or blue light. Light intensity enhanced the growth rate and concentration.

  11. Specific growth rate increased with temperature.

  12. Biomass concentration decreased with temperature.

  13. Biomass productivity increased first then declined.

  14. Blue light favored the protein synthesis. • Protein content had negative correlation with temperature. • Bradford protein assay

  15. Red light favored the lipid synthesis in the exponential phase. • Positive correlation between lipid content and temperature. • Nile-red staining lipid assay

  16. Light quality showed little effect on carbohydrate synthesis • A small increase observed with temperature. • Phenol-sulfuric acid assay

  17. Summary • Growth kinetics and Nutrient composition • culture temperature light quality light intensity • Acknowledgement • Dr. Tyler W. Johannes • The University of Tulsa • Russell School of Chemical Engineering • Indreesh Badrinarayanan • Questions?

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