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Brisbane

Sampling at bore source. Sampling below bore source. Brisbane. New Lorne Bore (RN 17263). Depth 1613 m, Temp: 89 o C, pH 8.5); Flow rate: 7.56 L/sec; Chemistry: typical Na-HCO 3 -Cl. The New Lorne Bore (RN 17263) runoff channel

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Brisbane

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  1. Sampling at bore source Sampling below bore source Brisbane New Lorne Bore (RN 17263). Depth 1613 m, Temp: 89 oC, pH 8.5); Flow rate: 7.56 L/sec; Chemistry: typical Na-HCO3-Cl Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS)

  2. The New Lorne Bore (RN 17263) runoff channel Temperature specific microbial colonisation occurs in the New Lorne bore run off channel in a temp gradient (88 oC to ambient) Sampling microbial mats Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS)

  3. Grey Filamentsat 75ºC Red Filamentsat 66ºC Green Filaments at 57ºC Brown Filamentsat 52ºC 4 spatially distinct colored microbial mats colonise New Lorne bore runoff channel at different temperatures Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS)

  4. 5 Samples (4 mats + source sample) HMW DNA extracted 16S rRNA genes cloned 400 clones studied to determine phylogroups Representative of each phylogroup sequenced Phylogenetic Reconstruction

  5. 2 1 4 3 6 Dictyoglomus Thermomicrobia 5 Thermodesulfobacteria Thermotoga Chrysiogenetes 7 Proteobacteria Aquifex Deferribacter Thermales Nitrospira Cyanobacteria Firmicutes 8   Verrucomicrobia 9    Acidobacteria Fibrobacter Planctomycetes Actinobacteria Chlamydia Microbial diversity of the New Lorne bore & the 4 runoff channel microbial mats (10 / 21 kingdoms) Fusiforms 10 Spirochetes Bacteroides

  6. Red Grey Green Brown Group-genus-species distribution (75ºC) (66ºC) (57ºC) (52ºC) Bacillus þ þ Hydrogenobacter þ þ Cytophagales þ Clostridia þ þ þ Thermus þ þ þ þ þ Meiothermus þ þ Planctomycetes þ þ þ Chloroflexus þ þ Cyanobacteria þ þ Acidobacteri þ a -Proteobacteria þ þ þ -Proteobacteria b þ þ -Proteobacteria g þ -Proteobacteria d þ Nitrospira þ Sporomusa þ Environental Clones þ þ Prosthecobacter The microbial diversity profile of the 4 microbial mats Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS)

  7. Genus / species Source Grey Red Green Brown Phylum (89 ºC) (75 ºC) (66 ºC) (57 ºC) (52 ºC) Aquificae 100 6 3 H. subterranea Deinococci 62 4 Thermus T. scotoductus 12 3 T. igniterrae 1 T. oshimai 47 4 Meiothermus M. ruber 2 34 M. cerberus 4 10 3 Chloroflexi C. auranticus 4 Nitrospira N. muscoviensis Verrucomicrobia 1 36 1 Cyanobacteria F. musicola 1 2 5 Planctomycetes 2 8 Acidobacteria 3 6 Bacteroidetes Proteobacteria a 1 20 - Proteobacteria b 13 2 - Proteobacteria d 2 - Proteobacteria 8 8 1 Firmicutes Novel, Unaffiliated?? 9 24 21 21 Quantitative diversity based on clones (in %)

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