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Lecture 16: DNA replication

Lecture 16: DNA replication. Announcements. Exam 2 grades, key are posted on the course website. Exam 2 average: 76. Lab report 2: due next week Final Exam: scheduled for Friday May 6 at 8:00 a.m. Want to reschedule?. Review of DNA structure. antiparallel strands.

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Lecture 16: DNA replication

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  1. Lecture 16: DNA replication

  2. Announcements Exam 2 grades, key are posted on the course website Exam 2 average: 76 Lab report 2: due next week Final Exam: scheduled for Friday May 6 at 8:00 a.m. Want to reschedule?

  3. Review of DNA structure antiparallel strands

  4. “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material…” Watson and Crick, 1953

  5. Three models for the mode of DNA replication

  6. The Meselson-Stahl experiment

  7. TheMeselson-Stahl experiment ruled out conservative mode ruled out dispersive mode

  8. DNA replication origins Eukaryotes: many origins per chromosome Bacteria: one single origin

  9. DNA polymerase catalyzes the chain-elongation reaction DNA polymerase

  10. Bacterial DNA polymerases

  11. DNA pol. III holoenzyme

  12. Replication initiation at E. Coli OriC

  13. DNA gyrase removes extra DNA “twists” during replication

  14. Initiation of DNA synthesis requires a primer

  15. Leading and lagging strand DNA synthesis replication fork

  16. Leading and lagging strands are synthesized simultaneously

  17. Summary of DNA replication in E. Coli

  18. DNA replication in eukaryotes:multiple origins

  19. DNA replication in eukaryotes:coordination with the cell cycle

  20. DNA replication in eukaryotes:multiple polymerases

  21. DNA replication in eukaryotes:dealing with nucleosomes nucleosomes

  22. DNA replication in eukaryotes:replicating the telomeres The problem: The solution: new lagging strand is shorter

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