1 / 10

Proteomics: 2D Gel

Proteomics: 2D Gel. Proteome analysis 장치 (2D). Root Proteome. Nodule Proteome. Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS. 단백질의 질량 스펙트럼. 효소. 질량분석기. MS spectrum. 단백질. Protein database. Peptide sequences. Protein id. A. B. A. B.

vahe
Download Presentation

Proteomics: 2D Gel

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Proteomics: 2D Gel

  2. Proteome analysis 장치 (2D)

  3. Root Proteome Nodule Proteome

  4. Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS

  5. 단백질의 질량 스펙트럼 효소 질량분석기 MS spectrum 단백질 Protein database Peptide sequences Protein id A B A B

  6. Proteomics: MALDI-Mass

  7. Mass spectrum vs. database Mass spectrum database Protein A Protein B Protein C

  8. 탄뎀 질량 스펙트럼(MS/MS spectrum) energy 효소 1st MS 2nd MS 단백질 MS spectrum Peptide sequence Protein id Protein database Tandem MS spectrum

  9. R1 O R2 O R3 O R4 COOH H2N C C N C C N C C N C H H H H H H H 탄뎀 질량 스펙트럼Peptide fragmentation x3 y3 z3 x2 y2 z2 x1 y1 z1 a1 b1 c1 a2 b2 c2 a3 b3 c3

  10. 2D data 분석

More Related