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Chapter 13

Chapter 13. Differential Gene Expression & plant development. Regulation of gene expression. Environmental stimuli Hormones Transcription factors microRNAs. Arabidopsis. MicroRNAs. Overexpression of miR156. microRNAs and RNAi. RNAi – RNA interference

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Chapter 13

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  1. Chapter 13 • Differential Gene Expression & plant development

  2. Regulation of gene expression • Environmental stimuli • Hormones • Transcription factors • microRNAs

  3. Arabidopsis

  4. MicroRNAs • Overexpression of miR156

  5. microRNAs and RNAi • RNAi – RNA interference • Used to determine the consequences of down-regulating gene expression

  6. Technologies used in gene identification • RNAi • Mutational studies • Transposon tagging • Microarray analysis

  7. Arabidopsis

  8. Transposable elements

  9. Transposable elements • AC and DS in maize • AC encodes transposase, required to excise DS

  10. Transposon tagging

  11. Transposon tagging utilizes colorimetric expression assays • GUS reporter gene (B-glucuronidase), E. coli • GFP (green fluorescent protein), jellyfish

  12. DNA microarrays • Can accommodate ~20,000 genes

  13. Microarrays and development • Example • Vegetative meristem mRNA labeled green • Flower bud mRNA labeled red

  14. The discovery of microRNAs has resulted in which gene analysis technology? • RNAi • Chemical mutagenesis • Transposon tagging • Microarray analysis

  15. GUS and GFP reporter genes are utilized in • RNAi • Chemical mutagenesis • Transposon tagging • Microarray analysis

  16. Comparing gene expression under two different conditions is best accomplished using • RNAi • Chemical mutagenesis • Transposon tagging • Microarray analysis

  17. Determining which tissues a gene is expressed in can be examined using • RNAi • Chemical mutagenesis • Transposon tagging • Microarray analysis • More than one of the above

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