1 / 29

Microbiology – Practical Review

Microbiology – Practical Review. Patricia Sidelsky - 2004. How do you make a smear from a solid culture and a broth culture?. Gram Stain. What is the primary stain ?. Crystal violet. What is the counterstain?. Saffranin. Gram positive organisms stain_____________because___________________

valterra
Download Presentation

Microbiology – Practical Review

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Microbiology – Practical Review Patricia Sidelsky - 2004

  2. How do you make a smear from a solid culture and a broth culture?

  3. Gram Stain What is the primary stain ? Crystal violet What is the counterstain? Saffranin

  4. Gram positive organisms stain_____________because___________________ Gram negative organisms stain _____________ because______________________________

  5. The most common errors in gram staining? • Decolorizer • Fixing • Timing • Smear

  6. What is the importance of using iodine in the Gram stain? Iodine is the mordant . It helps the crystal violet stick to the gram positive cell wall

  7. Morphology and Gram stain?

  8. Gram stain

  9. Blood agar plates What are the types of hemolysis?

  10. This is selective for…………………

  11. What organisms produces these results?Why?

  12. These results are caused by?

  13. EMB stands for What do these results show?

  14. Differential and selective ? What agar? Selects for? Differentiates between?

  15. Sugar fermentation Explain the results in tube 3A

  16. What is the agar used in this test? Explain the results in tube 4 Explain the results in tube 2 Explain the results in tube 5

  17. What enzyme causes the liquefaction of gelatin?

  18. What organism tests positive ? What causes the pink color?

  19. What enzyme changes the color from green to blue? Organisms that have a positive test use what for their source of carbon?

  20. What reagent is added to these tubes ?

  21. This test is used to differentiate between?

  22. This experiment is used to test for …………………….. What type of agar? Which is the positive result ? What is this reagent?

  23. Sensitive( susceptible) or resistant? Type of agar ?????????

  24. Test for what kind of organisms ?

  25. The name of the test is ……………….. What reagent is added to the slant? Which organism tests positive?

  26. What bacterium has a positive test?

  27. What two organisms are catalase ? What compound is broken down in the above reaction? What gas is produced?

  28. What bacterium ferments mannitol and has a high tolerance for salt? Do any other organisms tolerate salt?

More Related