1 / 21

Lecture 16

Lecture 16. Nucleic Acid Chemistry. Hydrolysis of RNA. Occurs under alkaline conditions. Hydrolysis of RNA. Nucleic Acid Chemistry. Denaturation (increase T). RNA-RNA>RNA-DNA RNA-DNA>DNA-DNA. Nucleotides Absorb UV Light. Base stacking decreases absorption Hypochromic effect.

vance
Download Presentation

Lecture 16

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Lecture 16 Nucleic Acid Chemistry

  2. Hydrolysis of RNA • Occurs under alkaline conditions

  3. Hydrolysis of RNA

  4. Nucleic Acid Chemistry Denaturation (increase T) RNA-RNA>RNA-DNA RNA-DNA>DNA-DNA

  5. Nucleotides Absorb UV Light • Base stacking decreases absorption • Hypochromic effect

  6. Denaturation

  7. Deamination

  8. Alkylation

  9. Depurination

  10. Pyrimidine Dimers

  11. DNA photolyase Native DNA uv light Pyrimidine dimer DNA photolyase binds ( Light (>300nm) Light used to correct dimer Enzyme releases repaired DNA

  12. DNA Sequencing • Mid 1970s • Alan Maxam and Walter Gilbert • Chemical cleavage method • Fred Sanger • Chain-terminator method • Dideoxynucleotides

  13. Maxam Gilbert DNA Sequencing 1. Cleave DNA strand at specific nucleotides -e.g. A only 2. Separate fragments according to size by electrophoresis

  14. Sanger Method • Simpler and more widely used • Uses DNA replication by DNA polymerase • All DNA polymerases • Require primer + template • Synthesize 53

  15. DNA Primer and Template

  16. ddNTP Analog

  17. Sanger Method

  18. Sanger Method

  19. Sanger Method Gel

  20. Automation of DNA sequencing

  21. Automation of DNA Sequencing

More Related