1 / 11

ELISA

ELISA. By: Daniel Liu Class: Biology Teacher: Simonof. The uses of this test:. Test for antigens Test for antibodies Test blood for allergen reaction antigens Test food for allergens Test blood for HCG. Testing for antigens/antibodies.

vanna
Download Presentation

ELISA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ELISA By: Daniel Liu Class: Biology Teacher: Simonof

  2. The uses of this test: • Test for antigens • Test for antibodies • Test blood for allergen reaction antigens • Test food for allergens • Test blood for HCG

  3. Testing for antigens/antibodies • In each scenario a micro titer plate is lined with antigens to test for antibodies and vise versa. • Testing for antigens is called an Direct ELISA test • Testing for antibodies is called an Indirect ELISA test ~Keep in mind the immune system releases fighting allies to get rid of intruders in your system. The allies are called antibodies and the intruders are antigens.

  4. *Color Change ~Rinsing after every step is important because it clear out the antigens/antibodies that don’t stick

  5. Testing food for allergens • In the case of food when a food that carries an allergen enters your body, your body sends out antibodies to attack them. • So whenever you eat that food it is basically like you are getting sick and your body is having a “war”everytime the food allergen comes enters your body.

  6. Foods made in a factory line are a huge problem because bits of other foods could be manufactured into the processed food. • This means you can accidently eat something you are allergic to without even knowing.

  7. Factories uses 2 different tests to see if the food allergen was picked up along the line: • ELISA test (enzyme-linked immunosorbent assay) • PCR (Polymerase chain reaction)

  8. ELISA vs PCR • ELISA: ~Pros: A cheap and quick way to test for the allergens (proteins) in foods ~Cons: Once food is heated the protein is changed and you can’t detect them anymore (Dangerous because the allergens can still cause harm to the consumers even if heated and undetected) • PCR: ~Pros: Very accurate in testing because it tests for the DNA of the allergens (The DNA is still intact after heating) ~Cons: Expensive and timely processes

  9. Testing for HCG • HCG is a pregnancy hormone that can be detected through test strips. • The 3 zones of the test strips are layers with antibodies that act differently depending on if HCG is in your urine. • There are two results in a HCG test: • Positive: HCG is found in your urine • Negative: No HCG present

  10. The 3 zones of test strip: The Reaction Zone, Test Zone, and Control Zone • ~The Test and Control Zone both have a red dye substrate • Positive result: The last two zones will have color change • - The HCG brings along the antibodies in the 1st zone and flows along. They attach to the last 2 zones and the enzymes activate the red dye substrate • Negative result: Only the last zone has color change • -The antibodies in the 1st zone is carried along and flows though the 2nd zone and attaches to the last zoneand the enzymes activates the red dye substrate

  11. Sources • http://www.sumanasinc.com/webcontent/animations/content/ELISA.html • http://bcs.whfreeman.com/stryer/cat_040/ch04/ch04xd06.htm • http://www.webmd.com/allergies/guide/blood-test • http://www.microbac.com/uploads/Technical%20Articles/pdf/ELISA%20and%20PCR%20Method%20Allergen%20Testing%20in%20Food%20Products.pdf • http://www.as.nchu.edu.tw/lab/5c/course/antibody/week%2010%20ELISA.pdf ~Thank you for listening and hope you enjoyed my PowerPoint

More Related