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Lab 2: Preparing to clone the RFP gene: digesting the pKAN -R and pARA

Lab 2: Preparing to clone the RFP gene: digesting the pKAN -R and pARA. Pre-lab activity. DNA isolation. Transform. Express. Clone. Purify. Lab 2: Preparing to clone the RFP gene: digesting the pKAN -R and pARA. Title goes at the top of the first page

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Lab 2: Preparing to clone the RFP gene: digesting the pKAN -R and pARA

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  1. Lab 2: Preparing to clone the RFP gene: digesting the pKAN-R and pARA Pre-lab activity DNA isolation Transform Express Clone Purify

  2. Lab 2: Preparing to clone the RFP gene: digesting the pKAN-R and pARA • Title goes at the top of the first page • Each section must be clearly underlined • Skip lines between each section • Use blue or black PEN! • Follow these directions. You must be ready for lab (including pre-lab questions) at the beginning of the period. • 1. Title: copy this title..." Preparing to clone the RFP gene: digesting the pKAN-R and pARA ” • 2. Background: write a definition for the following words: plasmids, gene expression, selectable markers, pARA, pKAN-R, RNA polymerase, beta lactamase, ampicillin, fluorescent protein, restriction site. • 3. Pre-lab questions: Answer the STOP and THINK question on page B-13. • 4. Objective: Read the background information and determine the objective of the lab. • 5. Hypothesis: Create your hypothesis. • 6. Materials: copy the materials in a bulletedlist • 7. Procedure: Create a flowchart of the procedure. • 8. Data : Take a picture of your tubes. Print and label. • 9. Data Analysis: Copy and answer the STOP & THINK questions on page B-15 & B-16. • 10. Conclusion: Copy & answer the 5 questions page B-17. (Do this after lab is over).

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