1 / 1

NEN_69_9_2010_07_02_KLEOPA_200674_SDC1

varen
Download Presentation

NEN_69_9_2010_07_02_KLEOPA_200674_SDC1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplemental Figure 1. Immunoblot analysis of neurofilaments (NFs) in sciatic nerve. A. Following tissue lysis with RIPA buffer, equal amounts of soluble (RIPA sol) and insoluble (RIPA insol) fractions were run in SDS-PAGE and blotted with SMI31 and SMI32. Only a very small fraction of either phosphorylated (SMI31) or dephosphorylated (SMI32) NFs remains in the RIPA-insoluble fraction. B. Comparison of different lysis buffers for immunoblot analysis of NFs. Sciatic nerve tissue was processed either with RIPA buffer without addition of phosphatase inhibitors (RIPA), with RIPA after adding phosphatase inhibitors (RIPA+PI), or with Leammli buffer adding phosphatase inhibitors (Laemmli+PI). Samples were run in SDS-PAGE and blotted with SMI31 and SMI32 as well as with GAPDH for loading control. No significant differences in regards to the addition or not of phosphatase inhibitors, or in regards to the lysis buffer (RIPA vs. Laemmli) are found in the levels of phosphorylated NF-H (200 kDa) and NF-M (160 kDa) recognized by SMI31, nor in the levels of dephosphorylated NF-H (200 kDa) recognized by SMI32.

More Related