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’ITR

a. ’ITR. ’ITR. Substrate.  (2.89 kbp).  (1.74 kbp). one double strand cleavage. a. Circular donor.  (3.04 kbp).   (1.59 kbp). two double strand cleavages. M. 0. 0.5. 1. 2. 4. 6. 8. 24 hours. kbp. 23.1. a + d. 3Tet3 (1.3 kbp). . . 9.4. Substrate. 6.5.

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’ITR

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  1. a. ’ITR ’ITR Substrate  (2.89 kbp)  (1.74 kbp) one double strand cleavage a. Circular donor  (3.04 kbp) (1.59 kbp) two double strand cleavages M 0 0.5 1 2 4 6 8 24 hours kbp 23.1 a + d 3Tet3(1.3 kbp)   9.4 Substrate 6.5 4.3 b+g 2.3 2 a + d Substrate 0.5 b. Linear donor M 0 0.5 1 2 4 6 8 24 hours kbp a + d 23.1 9.4 Substrate 6.5 4.3 b + g 2.3 2 a + d Substrate 3Tet3 0.5 d. Linear donor Circular donor

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