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Phytoplankton in Six New York Lakes. Arbutus, Catlin, Green, Oneida, Onondaga, and Wolf. Presented by Thomas Clement & Amanda Stella. http://www.nies.go.jp/biology/mcc/images/PCD5008/0998L.jpg. http://www.imagequest3d.com/photos/phytoplankton/s003.jpg. Why are phytoplankton important?.
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Phytoplankton in Six New York Lakes Arbutus, Catlin, Green, Oneida, Onondaga, and Wolf. Presented by Thomas Clement & Amanda Stella http://www.nies.go.jp/biology/mcc/images/PCD5008/0998L.jpg http://www.imagequest3d.com/photos/phytoplankton/s003.jpg
Why are phytoplankton important? • Produce O2 in lakes • Indicator of primary production • Control Other populations of organisms http://www.umanitoba.ca/institutes/fisheries/227_305.jpg
How phytoplankton is measured • Sampling Techniques • Van Dorn • Preservation with Lugols solution • Lab Techniques • Water settled for several days • Increased concentration • Identified to Genus http://www.fisheries.org/education/fisheries_techniques/Chapter4/Removal%20of%20a%20water%20sampler%20from%20a%20Van%20Dorn%20sampler.jpg
Chlorophyll • Main pigment in photosynthetic organisms • Commonly used as an indicator of production http://www.public.asu.edu/~jpbirk/3dstruct/chlorophyll.gif
Chlorophyll Analysis • Water sampled out in field • In Lab • Mix sample • Measure sample • Filtered • Packaged in foil • Frozen until analysis http://www.umass.edu/tei/mwwp/Images/Filt.gif&imgrefurl=http://www.umass.edu/tei/mwwp/chloro.html&h=267&w=247&sz=6&hl=en&start=71&tbnid=DZS0KGvprZR8wM:&tbnh=113&tbnw=105&prev=/images%3Fq%3Dchlorophyll%26start%3D60%26ndsp%3D20%26svnum%3D10%26hl%3Den%26lr%3D%26safe%3Doff%26client%3Dfirefox-%26rls%3Dorg.mozilla:en-US:official%26sa%3DN
Chlorophyll Analysis Con’t • 90% buffered acetone used for extraction • Filters added to 15ml alkaline acetone solution • Refrigerated for 24 hours • Fluorometer was used • Fluorometer produces a concentration in µgrams/liter http://cacique.uprm.edu/gers/fluorometer.jpg
Conclusions • Cross-Lake Comparisons • Wide variety of phytoplankton • Sampling Error • Sampling day conditions • Analyzing techniques