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Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion

Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion. Genti Gjyzeli Kelly Mitok Corey O’Reilly. CIP4 Structure. SH3. SH3. FBAR. HR1. HR1. FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009.

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Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion

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  1. Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion Genti Gjyzeli Kelly Mitok Corey O’Reilly

  2. CIP4 Structure SH3 SH3 FBAR HR1 HR1 FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

  3. CIP4 is involved in Endocytosis Pitts, 2005 Shimada et al., 2007 FBAR and HR1 domains FBAR domain FB

  4. Membrane Interacting Residues in Endocytosis Intracellular Extracellular Shimada et al., 2007

  5. CIP4 in Endocytosis Intracellular Extracellular FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

  6. Overexpression of CIP4 Overexpression of CIP4 in neurons gives large protrusion Normal Cortical Neuron Microtubule CIP4 Actin Microtubules Actin Pictures provided by Dent and Saengsawang

  7. CIP4 Location in Growth Cone Actin CIP4 Video provided by Dent and Saengsawang

  8. CIP4 Location in Growth Cone Actin CIP4 Video provided by Dent and Saengsawang

  9. Residues that may be important in protrusion Intracellular Extracellular Shimada et al., 2007

  10. CIP4 in Protrusion Intracellular Extracellular FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

  11. Future Mutation Studies to Determine Important Residues in Protrusion Shimada et al., 2007

  12. Future Studies to analyze Potential Phosphoregulation of CIP4 Roberts-Galbraith et al., 2010

  13. Diseases Associated with CIP4 • Huntington’s Disease • Cancer Joshi, 2010

  14. Biocore Instructional Materials • Learning Goals: • Predict how domains of a protein can influence it’s overall function • Electrostatic interactions of protein with membrane • One protein  one function • Predict protein function from location in cell • Endocytosis • Materials: • Jmol tutorial • Cellular landscape • Animation

  15. Thanks Michelle Harris Erik Dent Witchuda Saengsawang Tim, Mark, and Margaret at CBM of MSOE The other UW-Madison team for their feedback

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