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The Endomembrane System

The Endomembrane System. Text and image sources are included using the notes function of the .ppt file. Cellular Membranes. Pulse-Label Autoradiography. 7 minutes. 3 minutes. Pulse-Label Autoradiography. 37 minutes. 117 minutes. The Endoplasmic Reticulum. DiOC6(3). ER dynamics. Video.

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The Endomembrane System

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  1. The Endomembrane System Text and image sources are included using the notes function of the .ppt file

  2. Cellular Membranes

  3. Pulse-Label Autoradiography 7 minutes 3 minutes

  4. Pulse-Label Autoradiography 37 minutes 117 minutes

  5. The Endoplasmic Reticulum DiOC6(3)

  6. ER dynamics Video ER / Mitochondria

  7. “Smooth”ER

  8. Abnormal Pathology sER from a chondrocyte of a patient with pseudoachondroplasia

  9. Transition zones SER RER

  10. The rER

  11. Supplying the NE

  12. Movin’ on Note points of vesicle formation

  13. Vesicular Traffic There are at least several (probably many more) distinct classes of vesicle coats/sorting mechanisms that function at different places in the cell

  14. coatomer-,smooth-coated vesicles

  15. Biochemical isolation of clathrin- coated vesicles

  16. Clathrin Animation

  17. Dynamin Breakin’ up is hard to do 200 nm 100 nm shits wt

  18. Fusion Just as there are specific mechanisms for sorting and vesicle formation, there are equally specific mechanisms for vesicle delivery

  19. VSVGts::GFP transport Video Vesicular stomatitis virus glycoprotein, temp-sensitive folding mutant

  20. The Golgi

  21. Perinuclear Golgi

  22. Biochemical stratification

  23. Post-Golgi transport tubules Video

  24. Coatamer I Dynamics at the Golgi Fluorescence Recovery After Photobleaching (FRAP) of COPI::GFP

  25. Lysosomes

  26. Exocytosis/Endocytosis

  27. Peroxisomes

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